摘要： 为克服传统方法制备单克隆抗体复杂而费时的弊病，研究介绍了一种从多克隆抗体库中快速分离高特异性抗体的方法；根据抗原抗体特异性结合的原理，以大肠杆菌系统表达的甜菜夜蛾围食膜蛋白SeP89为抗原，从多克隆抗体库中分离其特异性抗体；成功从甜菜夜蛾围食膜蛋白抗血清库中分离SeP89蛋白的特异性抗体；以快速分离围食膜蛋白SeP89抗体为例，研究建立了一种快速从多克隆抗体库中分离特异性抗体的方法。

关键词: 抗体, cDNA克隆, 围食膜蛋白, 抗血清, Western blot

Abstract: The common method for monoclonal antibody preparation is complex and time-consuming.So a procedure which allowed rapid and efficient isolation of target protein from a library of antibodies was reported here.According to immunology protocol,specific antibodies to a peritrophic membrane protein,SeP89(which was expressed in Escherichia coli),was isolated from a polycolonalantiserum against a pool of proteins from the PM of Spodoptera exigua.We successfully isolated highly specific antibodies to SeP89 protein from a polycolonal antiserum pool.A method of rapid and efficient isolation of highly specific antibodies from an antiserum against a pool of proteins was established.

Key words: Antibody, cDNA clone, PM protein, Antiserum, Western blot

中图分类号: 

• Q511