摘要： 以波尔山羊体细胞作为核供体进行核移植,比较受体卵母细胞来源以及不同供体细胞类型对核移植重构胚融合率的影响。结果:以体内成熟和FSH处理卵巢、屠宰场卵巢来源的体外成熟卵母细胞做核移植受体卵母细胞,重组卵的融合率分别为71.9%,78.4%,73.1%,三者之间差异不显著;以卵丘细胞和皮肤成纤维细胞做核供体,重组卵的融合率分别为77.1%,68.3%,二者之间差异显著。
关键词:
山羊,
核移植,
供体细胞,
卵母细胞,
电融合
Abstract: The aim of the present study was to carry out nuclear transfer with the goat somatic cell as nuclear donors,to compare the effects of the sources of recipient oocytes and donor cell types on the fusion rate of reconstructed oocytes.The result showed that the fusion rate of reconstructed oocytes from in vivo or in vitro matured oocytes which was FSHstimulated or abattoir derived was 71. 9%,78. 4%,73. 1%respectively, there was no significant difference among them.Cumulus cells and skin fibroblast cellswas used as donor cells,and the fusion rate of reconstructed oocytes from them was77. 1%, 68. 3%respectively,there was significant difference between them.
Key words:
Goat,
Nuclear transfer,
Donor cell,
Oocytes,
Electric fusion
中图分类号:
刘海军, 刘灵, 刘玉堂, 李平, 池炳杰. 受体卵母细胞来源和供体细胞类型对山羊体细胞核移植融合率的影响[J]. 华北农学报, 2008, 23(1): 41-44. doi: 10.7668/hbnxb.2008.01.010.
LIU Hai-jun, LIU Ling, LIU Yu-tang, LI Ping, CHI Bing-jie. The Effect of Recipient Oocytes Source and Donor Cells Type on the Fusion Rate of Reconstructed Oocytes in Goat Somatic Cell Nuclear Transfer[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(1): 41-44. doi: 10.7668/hbnxb.2008.01.010.