摘要： 以口蹄疫病毒株OA/58 RNA为模板, 反转录并扩增目的cDNA, 然后与pGEM-TEasy载体连接并转化JM109菌株, 提取的重组质粒用凝胶电泳、PCR和EcoRⅠ酶切法鉴定。该毒株与Poliovirus, Hepatitis Avirus和Human rhi-novirus 89毒株3C序列对比分析发现核苷酸序列一致性分别为38.8%, 37.1%和36.5%, 氨基酸序列一致性分别为23.7%, 19.2%和17.6%。通过Swiss-pdbViewer软件模拟出FMDV OA/58 3C蛋白酶的3D结构和表面模型。并鉴定出此毒株3C蛋白酶的活性中心为Cys₃₁-His₄₆-Asp₈₄。

关键词: 口蹄疫病毒, 3C蛋白酶, 活性位点

Abstract: Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR.The amplified cDNA products were cloned into pGEM-T Easy Vectors and transformed into JM 109. The recombinant plasmids were identified by electrophoresis, PCR, and Eco R I cleavage. The nucleotide and amino acid sequences were compared with the 3C sequences of poliovirus, Hepatitis A virus and Human rhinovirus 89. The sequences alignments revealed that the agreement of OA/ 58 3C sequence to three reference viruses are 38.8%, 37. 1%, 36.5% at the nucleotide level, 23. 7%, 19. 2%, 17.6% at the acid level. By means of Swiss-pdbV iewer software, the 3D and surface mold of FMDV OA/58 3C protease was ohtained. In addition, Cys₃₁-His₄₆-Asp₈₄ may he an active site in FMDV、OA/58 3C protease.

Key words: FMDV, 3C protease, Active site

中图分类号: 

• S852.65

• Q78