水稻黑条矮缩病毒基因组片段6编码一种非结构蛋白

doi:10.7668/hbnxb.2007.06.002

华北农学报 ›› 2007, Vol. 22 ›› Issue (6): 5-8. doi: 10.7668/hbnxb.2007.06.002

所属专题： 水稻；生物技术；

水稻黑条矮缩病毒基因组片段6编码一种非结构蛋白

方守国¹, 王朝辉¹, 韩成贵^{1, 2}, 李大伟¹, 于嘉林¹

1. 中国农业大学, 农业生物技术国家重点实验室, 北京, 100094;
2. 中国农业大学, 植物病理学系, 北京, 100094

收稿日期:2007-02-05 出版日期:2007-12-28
通讯作者: 韩成贵(1966-), 男, 辽宁法库人, 博士, 教授, 主要从事植物病毒学研究
作者简介:方守国(1965-), 男, 湖北天门人, 博士, 副教授, 主要从事分子病毒学研究
基金资助:
国家转基因植物研究与产业化专项(J-A-027); 国家“973”课题(2006CB101903)

Genomic Segment 6 of Rice black-streaked dwarf virus Encodes for a Viral Non-structural Protein

FANG Shou-guo¹, WANG Zhao-hui¹, HAN Cheng-gui^{1, 2}, LI Da-wei¹, YU Jia-lin¹

1. State Key Laboratory for Agrobiotechnology, Beijing 100094, China;
2. Department of Plant Pathology, China Agricultural University, Beijing 100094, China

Received:2007-02-05 Published:2007-12-28

摘要/Abstract

摘要： 水稻黑条矮缩病毒(Rice black-streaked dwarfvirus, RBSDV)是引起我国水稻黑条矮缩病和玉米粗缩病的病原。引起玉米粗缩病的RBSDV湖北分离物10条基因组dsRNA全序列已被确定, 其中基因组片段6(S6)全长序列为2645bp, 只含有一个阅码框(82~2460 nt), 编码一个分子量约为89.6 kDa的蛋白(P6)。为进一步研究该蛋白的生物学功能, 在大肠杆菌中表达了RBSDV基因组片段6编码的P6蛋白并制备了相应的抗血清。Western blotting分析显示, P6抗血清不与纯化的RBSDV粒子蛋白发生反应, 而在被RBSDV感染的玉米叶片和带毒的传播介体---灰飞虱中可检测到P6。此结果表明, RBSDV-S6编码的蛋白是一种非结构蛋白。

关键词: 水稻黑条矮缩病毒, 基因组片段6, 原核表达, 非结构蛋白

Abstract: Race blade-streaked dwarf virus(RBSDV) can cause both maize rough dwarf and rice black streaked dwarf diseases in China. The complete sequences of all ten genomic segments(S1 to S10)from an isolate of RBSDV causir)rough dwarf disease on maize in Hubei Province had been determined. The S6 is 2645 base pairs in length and contains a single long open reading frame(ORF) encoding a putative 89.6 kDa protein(P6).In order to define the function of this protein, P6 was expressed in Escha-achia coli cells and antisermn against P6 was successfully prepared. In Western bloting analysis, P6 was specifically detected in the RBSD-infeded maize leaves and the viruliferous planthoppers(Laodelphax striatellus), while anti-P6 antisera did not read with any protein from purified RBSDV particles. These results suggested that RBSD-S6 encoded a viral nonstructural protein.

Key words: Race blacl-Streaked dwarf virus, Genomic segment 6, Expression, Nonstructural protein

中图分类号:

S432.41
Q78

方守国, 王朝辉, 韩成贵, 李大伟, 于嘉林. 水稻黑条矮缩病毒基因组片段6编码一种非结构蛋白[J]. 华北农学报, 2007, 22(6): 5-8. doi: 10.7668/hbnxb.2007.06.002.

FANG Shou-guo, WANG Zhao-hui, HAN Cheng-gui, LI Da-wei, YU Jia-lin. Genomic Segment 6 of Rice black-streaked dwarf virus Encodes for a Viral Non-structural Protein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 5-8. doi: 10.7668/hbnxb.2007.06.002.

http://www.hbnxb.net/CN/Y2007/V22/I6/5

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水稻黑条矮缩病毒基因组片段6编码一种非结构蛋白

Genomic Segment 6 of Rice black-streaked dwarf virus Encodes for a Viral Non-structural Protein

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水稻黑条矮缩病毒基因组片段6编码一种非结构蛋白

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