玉米蛋白激酶ZmSPK1在转基因拟南芥中的定位

doi:10.3321/j.issn:1000-7091.2006.03.001

华北农学报 ›› 2006, Vol. 21 ›› Issue (3): 1-4. doi: 10.3321/j.issn:1000-7091.2006.03.001

所属专题： 玉米；生物技术；

• 论文 • 下一篇

玉米蛋白激酶ZmSPK1在转基因拟南芥中的定位

肖雪^1,2, 张秀海², 杨清¹, 邹华文^1,2, 吴忠义², 于荣³, 曹鸣庆², 黄丛林²

1. 南京农业大学生命科学学院, 江苏南京 210095;北京市农林科学院北京农业生物技术研究中心, 北京 100089;
2. 北京市农林科学院北京农业生物技术研究中心, 北京 100089;
3. 南京农业大学生命科学学院, 江苏南京 210095;
4. 首都师范大学生命科学学院, 北京 100037

收稿日期:2006-03-20 出版日期:2006-06-28
通讯作者: 黄丛林
作者简介:肖雪（1980- ），女，江西吉安人，在读硕士，主要从事植物抗逆分子生物学方面研究工作。
基金资助:
国家基金（3040228）；北京市科委合同项目（H012010240240113）；北京市科委北京农业育种基础研究创新平台资助

Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis

XIAO Xue^1,2, ZHANG Xiu-hai², YANG Qing¹, ZOU Hua-wen^1,2, WU Zhong-yi², YU Rong³, CAO Ming-qing², HUANG Cong-lin²

1. College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;
2. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China;
3. College of Life Science, Capital Normal University, Beijing 100037, China

Received:2006-03-20 Published:2006-06-28

摘要/Abstract

摘要： 为了研究玉米蛋白激酶ZmSPK1在植物体内亚细胞水平上的分布情况，利用绿色荧光蛋白基因gfp为报告基因，分别构建了以35S为启动子表达ZmSPK1-GFP融合蛋白和GFP的植物表达载体35S：：ZmSPK1-GFP和35S：：GFP，用花粉浸醮的方法将表达载体转入拟南芥，筛选稳定表达株系，在共聚焦激光扫描显微镜下观察ZmSPK1在植物细胞中的定位。研究结果表明：融合蛋白ZmSPK1-GFP的荧光分布与游离GFP相似，在细胞浆及细胞核内均可见。与单独表达GFP相比，融合蛋白ZmSPK1-GFP在核内信号更强，推测ZmSPK1是可溶性蛋白，定位在核内。

关键词: ZmSPK1, 绿色荧光蛋白, 共聚焦激光扫描显微镜, 亚细胞定位

Abstract: To determine the subcellular localization of ZmSPK1,a protein kinase from maize,using 35S promoter we constructed two plant expression plastids:35S::ZmSPK1GFP and 35S::GFP,and transformed them respectively into Arabidopsis by floral dip.Subcellular localization was determined by Confocal laser scanning microscopy.The fused ZmSPK1-GFP showed a nuclear and cytosolic distribution similar to that of free GFP.Compared with 35S::GFP,ZmSPK1GFP was more strongly accumulated in the nucleus,suggesting that ZmSPK1 might be soluble protein and localize in the nucleus.

Key words: ZmSPK1, GFP, Confocal laser scanning microscope (CLSM), Subcellular localization

中图分类号:

S188

肖雪, 张秀海, 杨清, 邹华文, 吴忠义, 于荣, 曹鸣庆, 黄丛林. 玉米蛋白激酶ZmSPK1在转基因拟南芥中的定位[J]. 华北农学报, 2006, 21(3): 1-4. doi: 10.3321/j.issn:1000-7091.2006.03.001.

XIAO Xue, ZHANG Xiu-hai, YANG Qing, ZOU Hua-wen, WU Zhong-yi, YU Rong, CAO Ming-qing, HUANG Cong-lin. Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 1-4. doi: 10.3321/j.issn:1000-7091.2006.03.001.

http://www.hbnxb.net/CN/Y2006/V21/I3/1

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玉米蛋白激酶ZmSPK1在转基因拟南芥中的定位

Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis

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