摘要： 植酸酶是催化植酸及其盐类物质水解成肌醇和磷酸的一类酶的总称，用其作为动物饲料添加剂既可以达到提高磷利用率的目的，又可以降低环境中的磷污染。真正具有开发价值的是利用微生物生产植酸酶。直接以宇佐美曲霉菌株2418基因组DNA为模板，对植酸酶基因phyA的成熟肽（分子长度为1347 bp）进行了PCR扩增，再将PCR产物克隆入pBS-T载体，经DNA测序鉴定，与已发表的植酸酶基因同源性达到99%以上，这为进一步获得大量、高活性植酸酶以及研究和开发新型微生态制剂打下了基础。

关键词: 植酸酶, 克隆, 宇佐美曲霉

Abstract: The phytase gene(phyA) of Aspergillus usamii 2418 strain,which had been already selected and identified by us in the key laboratory of biomechanics and tissue engineering,was amplified by polymerase chain reaction(PCR) in this study.The PCR fragments were subcloned into the pBS-T vector.Nucleotide sequence analysis of the phyA gene showed that it comprised 1 347 bp without the signal peptide sequence and shows over 99% homology in nucleotides.It is the base of obtaining high active phytase and developing new microecological preparation.

Key words: Phytase, Cloning, Aspergillus usamii

中图分类号: 

• Q556