华北农学报 ›› 2005, Vol. 20 ›› Issue (6): 84-87. doi: 10.3321/j.issn:1000-7091.2005.06.022

所属专题: 番茄 蔬菜专题

• 论文 • 上一篇    下一篇

拮抗BS-208菌株对番茄灰霉病诱导抗性的初步研究

杜立新1, 冯书亮1, 王容燕1, 王金耀1, 曹伟平1, 林开春2, 张用梅2   

  1. 1. 河北省农林科学院植物保护研究所, 河北保定 071000;
    2. 武汉天惠生物工程公司, 湖北武汉 430070
  • 收稿日期:2005-08-13 出版日期:2005-12-28
  • 作者简介:杜立新(1978- ),男,河北武安人,硕士,主要从事植物病害生物防治研究工作。
  • 基金资助:
    河北省农林科学院重点项目(A03-1-03-09);武汉天惠生物工程公司合作研究项目资助

Preliminary Study on Induced Resistance of Antagonistic BS-208 to Tomato Grey Mould

DU Li-xin1, FENG Shu-liang1, WANG Rong-yan1, WANG Jin-yao1, CAO Wei-ping1, LIN Kai-chun2, ZHANG Yong-mei2   

  1. 1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China;
    2. Nature's Favour Bioengineering CO., LTD, Wuhan 430070, China
  • Received:2005-08-13 Published:2005-12-28

摘要: 本文对拮抗BS-208菌株防治番茄灰霉病的作用机制进行了初步研究。通过在番茄苗上接种番茄灰霉病菌和拮抗枯草芽孢杆菌BS-208,测定PAL、SOD、POD和PPO的活性变化及MDA含量。结果表明,接种BS-208菌株发酵液处理48h后PAL、POD和PPO活性均升高,其中PAL和POD活性在处理后48h达到最大值,均为0.72,PPO活性在处理后96 h达到最大值,为4.5,这些酶活性的升高增强了番茄抵御病原菌侵入的能力;而MDA含量与对照相比无明显变化,表明接种BS-208菌株后对番茄植株没有伤害。

关键词: 枯草芽孢杆菌, 番茄灰霉病, 诱导抗性

Abstract: Action mechanism of antagonistic BS-208 on tomato grey mould was studied.The activity changes of PAL,SOD,POD,PPO and content of MDA were tested by inoculating Botrytis cinerea and antagonistic BS-208 on tomato plants.The results showed that the activity of PAL,POD and PPO increased after inoculating strain BS-208 fermentation liquid.Among them,maximum activity of PAL and POD reached at 48 hours after the treatment were both 0.72(U),whereas,maximum activity of PPO reached at 96 hours after the treatment was 4.5(U).This increased resistance of tomato to pathogen.Moreover,the content of MDA were not significantly changed after treatment with BS-208,it indicated that tomato plants were not damaged by BS-208

Key words: Bacillus subtilis, Tomato grey mould, Induced resistance

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引用本文

杜立新, 冯书亮, 王容燕, 王金耀, 曹伟平, 林开春, 张用梅. 拮抗BS-208菌株对番茄灰霉病诱导抗性的初步研究[J]. 华北农学报, 2005, 20(6): 84-87. doi: 10.3321/j.issn:1000-7091.2005.06.022.

DU Li-xin, FENG Shu-liang, WANG Rong-yan, WANG Jin-yao, CAO Wei-ping, LIN Kai-chun, ZHANG Yong-mei. Preliminary Study on Induced Resistance of Antagonistic BS-208 to Tomato Grey Mould[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(6): 84-87. doi: 10.3321/j.issn:1000-7091.2005.06.022.