摘要： 对影响青麻叶大白菜游离小孢子培养的因素进行了研究.结果表明:供体植株的基因型与小孢子胚胎发生密切相关;33℃, 2 h高温处理有助于小孢子转化成胚状体, 每蕾成胚数比25℃恒温培养提高0.5倍;在培养基中加入外源激素和多种氨基酸可以提高胚状体的诱导效率, 平均比对照增加59.6%;培养基的琼脂含量增加到12g/L能显著提高小孢子胚的成苗率, 比含琼脂8g/L的高出0.5个百分点.通过对青麻叶大白菜小孢子胚的培养, 获得二倍性的双单倍体植株, 从中选出性状优良的纯系进行杂交组合的选配, 培育出青麻叶大白菜的新品系.

关键词: 游离小孢子培养, 大白菜, 基因型, 培养条件, 育种

Abstract: The affecting factors and cultural condition of the isolated microspore in Qingmaye Chinese cabbage breeding have been studied. The embryo formation is closely related with genotypes of donor plant High temperature treatment 33℃, 24 h can accelerate formation of embryo from microspore, the number of embryo per bud increased 4.5 times as much as that of 25℃ constant T. The added exogenous hormone and poly amino acid to culture medium can improve the inductivity of embryoid, increasing averagely 59.64% than that of the cont rast. The content of agar increased to 12 g/ L in culture medium can raise seedling rate of embryos significantly, 40.5% higher than that of 8 g/ L. Double haploid (DH) plant of Qingmaye Chinese cabbage was obtained through microspore culture. Isogonics lines with good characters in DH lines were selected to make up hybridized combinations, and a new variety of Qingmaye Chinese cabbage was bred.

Key words: Isolated microspore culture, Genotype, Chinese cabbage, Cultural condition, Breeding

中图分类号: 

• S63.103.6