摘要： 将猪流行性腹泻病毒(PEDV)免疫原免疫产蛋母鸡,收集卵黄提纯制备高效价卵黄抗体,作为治疗剂应用于试验感染腹泻仔猪,结果显示卵黄抗体具有显著的治疗效果,治疗组腹泻症状明显减轻,多数在5d后恢复正常,死亡率仅有16.7%;而对照组呈水样腹泻,最后脱水衰竭而死,死亡率为100%,两者差异极显著。
关键词:
猪流行性腹泻病毒,
仔猪腹泻,
卵黄抗体
Abstract: IgY against PEDV was obtained from egg yolk of chickens immunized with PEDV. Then I gY was puri fied by chloroform extrac tion and ammonium sulfate precipitation.After challenge exposures to PEDV, the neonatal piglets were treated orally wi th IgY solution in the antibodyt reatment group and with normal sal t solution in the control group af ter the occurrence of diarrhea.The mortali ty rate and the fecal scoring were used to evaluate the dif ference between the antibody treatment group and the control group. The experiment showed that the mortality rate was 100% in the cont rol group, whereas only 16. 7% in the ant ibody treatment group.The result indicates that IgY can be effectively used for piglet diarrhea.
Key words:
Piglet diarrhea,
PEDV,
IgY
中图分类号:
宋维平, 徐福洲, 王金洛, 杨兵, 赖平安, 孟彦妮. 抗猪流行性腹泻病毒卵黄抗体治疗效果研究[J]. 华北农学报, 2003, 18(1): 114-115. doi: 10.3321/j.issn:1000-7091.2003.01.032.
SONG Wei ping, XU Fu zhou, WANG Jin luo, YANG Bing, LAI Ping an, MENG Yan ni. Effect of IgY Treatment on Porcine Epidemic Diarrhea Virus(PEDV) in Piglets[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 114-115. doi: 10.3321/j.issn:1000-7091.2003.01.032.