摘要： 培养基中添加不同浓度的卡那霉素、头孢霉素和羧苄青霉素,观察抗生素对大白菜基因型GP-11种子发芽以及离体子叶再生的影响。结果表明:GP-11种子在含有 200mg/L卡那霉素的MS培养基上发芽生长时,幼苗子叶完全黄化,侧根数为0;随卡那霉素浓度的增加,芽、主根和侧根生长均受到一定程度抑制,但发芽率和发芽势不受影响.4日苗龄的离体子叶在含有 10mg/L卡那霉素并附加一定激素的改良MS培养基生长时,基本无绿芽诱出,在含75mg/L和 5mg/L卡那霉素的培养基中,诱出的绿芽率只有 32.5 %和 40.2%;在仅含3mg/L卡那霉素的生根培养基中,小苗完全丧失生根能力。在添加浓度高至400mg/L的头孢霉素或羧苄青霉素的诱芽培养基中,GP-11诱芽率为0,且它们之间差异不明显,但在添加头孢霉素或羧苄青霉素的生根培养基中,小苗生根依然正常。试验结果表明,在农杆菌介导的基因转化中,对大白菜转化苗筛选的卡那霉素浓度在10mg/L左右是适宜的,添加的抑菌剂浓度在能控制农杆菌生长的同时,尽量降低浓度,最好不超过400mg/L;在诱导生根培养基中,卡那霉素浓度不宜超过3mg/L.另外,对T0代转基因种子进行遗传分析时在发芽培养基中添加卡那霉素浓度应该达到200mg/L。

关键词: 大白菜, 卡那霉素, 头孢霉素, 羧苄青霉素, 发芽, 再生

Abstract: This paper studied the effect of the supplement of kanamycin, ceftaxime and carbenicillin on the germination of Chinese cabbage GP-11 seeds and in vitro regeneration of its cotyledon. The results showed that the seedling cotyledons completely became yellow and the number of lateral root was approximately zero when the concentration of kanamycin on germination media was raised to 200 mg/L.With the supplement of kanamycin,shoot, main root and lateral roots of GP-11 were inhibited. However, the germination rate and germination percentage did not change.No green shoot can be reduced when cotyledons of 4 days seedling were cultured in reformation M S media which was supplemented with 10 mg/L kanamycin and some hormone,and percentage of green shoot wad only 32.5%and 40.2% when the concentration of kanamycin was 7.5 mg/L and 5 mg/L,respectively.Little shoot completely lose the ability to root when kanamycin concentration in root media was only 3 mg/ L.In shoot-induced media added with 400 mg/L ceftaxime or carbenicillin, the percentage of shoot induction was zero,and there was no difference between ceftaxime and carbenicillin. Small shoot can normally root when cultured in media attached different concetraticm of ceftaxime and carhenicillin.

Key words: Chinese cabbage, Danamycin, Ceftaxime, Carbenicillin, Germination:Regeneration

中图分类号: 

• S634.1