华北农学报 ›› 2001, Vol. 16 ›› Issue (3): 35-39. doi: 10.3321/j.issn:1000-7091.2001.03.008

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芦苇愈伤组织对渗透胁迫和ABA的若干生理响应

吴诗光1, 陈龙1, 刘怀攀1, 张承烈2   

  1. 1. 周口师范高等专科学校生物系, 河南周口 466000;
    2. 兰州大学生命科学学院, 甘肃兰州 730000
  • 收稿日期:2001-05-15 出版日期:2001-09-28
  • 作者简介:吴诗光(1958- ),男,副教授,主要从事植物生理生化教学与研究工作。
  • 基金资助:
    国家自然科学基金资助项目(79570075)

Some Physiological Response of the Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA

WU Shiguang1, CHEN Long1, LIU Huaipan1, ZHANG Chenglie2   

  1. 1. Department of Biology, Zhoukou Normal College, Zhoukou Henan 466000, China;
    2. College of Life Sciences, Lanzhou Univesity, Lanzhou Gansu 730000, China
  • Received:2001-05-15 Published:2001-09-28

摘要: 研究了沙生芦苇胚性愈伤组织(SREC)和水生芦苇胚性愈伤组织(WREC)对PEG-6000的胁迫及外源ABA的生理响应。结果显示:10%~30%的PEG胁迫使2种愈伤组织的游离脯氨酸(Pro)含量升高、质膜透性增大、提高了保护酶(SOD、CAT、POD)的活性,降低了细胞相对含水量(RWC),SREC的游离Pro含量和保护酶活性的升高幅度大于WREC,质膜透性和RWC的变化前者小于后者。外源ABA对WREC的作用大于SREC。

关键词: 生态型芦苇, 胚性愈伤组织, 渗透胁迫, ABA, 生理生化特性

Abstract: We've studied the physiological changes of the embryogenic calluses of sand reed(SREC) and the water reed(WREC) in response to the osmotic stress of PEG 6000 and exogenous ABA.The results showed that the contents of free proline,relative membrane permeability and safeguard enzyme(CAT,POD and SOD)activity of SREC and WREC increased under the PEG osmotic stress treatment from 10%~30%,while the relative water content(RWC) decreased under the same treatment.Still the contents of proline and safeguard enzyme activity of SREC increased higher than these of WREC While the relative membrane permeability and RWC of SREC decreased less than these of WREC.It also showed the response of WREC to exogenous ABA was much higher than that of SREC.

Key words: Reed ecotypes, Enbryogenic callus, Osmotic stress, Exogenous ABA, Physiological andbiochemical response

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引用本文

吴诗光, 陈龙, 刘怀攀, 张承烈. 芦苇愈伤组织对渗透胁迫和ABA的若干生理响应[J]. 华北农学报, 2001, 16(3): 35-39. doi: 10.3321/j.issn:1000-7091.2001.03.008.

WU Shiguang, CHEN Long, LIU Huaipan, ZHANG Chenglie. Some Physiological Response of the Calluses from Reed Ecotypes to Osmotic Stress and Exogenous ABA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 35-39. doi: 10.3321/j.issn:1000-7091.2001.03.008.

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