鉴定菜豆品种的电泳方法

doi:10.3321/j.issn:1000-7091.1986.01.001

摘要/Abstract

摘要： 应用非变性的梯度聚丙烯酰胺凝胶电泳、SDS线性梯度聚丙烯酰胺凝胶电泳、聚丙烯酰胺和琼脂糖凝胶电聚焦分析了种子形态近似但茎生长习性不同（一个直立、一个缠绕）的两个菜豆品种的种子蛋白，结果表明它们的种子蛋白在电荷和分子量组成上都有显著差别。尤其以SDS电泳谱差别最大，种子系由北京市种子公司提供。总共33种亚基，两个品种共有的仅13个左右。因此我们提议进行菜豆品种鉴定，最好用SDS线性梯度聚丙烯酰胺凝胶电泳。本文首次报道菜豆种子蛋白有高分子量亚基存在。在较低分子量范围（10-25千道尔顿）存在变异，并可用在品种鉴定上。

关键词: 菜豆, 品种鉴定, 电泳方法

Abstract: Seed proteins of two cultivars of common bean(Phaseolus vulgaris)which share same seed morphology but different habit of shoot(one is erect,another is voluble)were analysed by using non-denaturating gradient polyacrylamide gel electrophoresis,SDS gradient polyacrylamide gel electrophoresis,polyacrylamide and agarose gel electric focusing.A considerable difference of both electric charge and molecular weight of seed proteins between two cultivars has leen found,especially,in protomers shown by SDS electrophoresis.Based on the fact that only 13 subunits are shared by two cultivars among total 33 bands,we suggested that SDS gradient polyacrylamide gel electrophoresis may be the most valuable in cultivar identification of common bean.Some new subunits which have their molecular weight higher than 100K and a variation occured in low molecular weight bands in bean proteins have been reported.

胡志昂, 王洪新. 鉴定菜豆品种的电泳方法[J]. 华北农学报, 1986, 1(1): 1-7. doi: 10.3321/j.issn:1000-7091.1986.01.001.

Hu Zhiang, Wang Hongxin. Different electrophoretic methods for cultivar characterization of common bean (Phaseolus vulgaris)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1986, 1(1): 1-7. doi: 10.3321/j.issn:1000-7091.1986.01.001.

http://www.hbnxb.net/CN/Y1986/V1/I1/1

参考文献

[1] Adriaanse et al.(1969):Characterization of Phaseolus vulgaris cultivars by their electrophoretic pattern.J.Sci.Food Agric.20;647-650.
[2] Beachy et al.(1981): Biosynthesis of subunits of the Soybean 7S storage protein.J.Mol.Appl.Genetics 1:19-27.
[3] Brown et al.(1980): Micro heterogeneity of G1 storage Protein from French bean with isoelectrofocusing.Pl.Physiol.66:838-840.
[4] Brown et al.(1982a): Genetic variation in the subunits of globulin-1 storage protein of French bean.Theor.APPl.Genet.59.83-88.
[5] Brown et al.(1981b): Genetic variation in the subunits of globulin-2 and albumin of French bean.Theor.Appl.Genet.60: 245-25O.
[6] Brown et al.(198lc): Linkage relationships between genes controlling seed proteins in French bean.Theor.Appl.Genet.60.251-259.
[7] Cooke(1984): The characterization and identification of crop cultivars by electrophoresis.Electrophoresis 5: 59-72.
[8] Davis et al.(1964): Disc electrophoresis.Method and application to serum proteins.Annals N.Y.Acad.Sci.121:404-427.
[9] Derbyshire et al.(1981): Protein profiles of Brazflfan beans(Phaseolus vulgaris) obtaines by electrophoresis in slabs of polyacrylamide gel.Energ.Nucle.Agric.3:100-109.
[10] Klozova.E.et al.(1983): Seed Protein characters in the study of Interand Intra specific velationships within the Phaseoleoe.(Fobaceae) in "Proteins and Nucleic Acids in Plant Systematics" eds: Jensen,U et D.E.Fairbrothers,P.341-351.Springer-Verlag.
[11] Langridge et al.(1981): Biochemieal genetics of some seed proteins of Pinus radiata.Bioehem.Geneties 19: 585-597.
[12] Muller(1984): Genotype-special Protein and isozyme patterns after direct isoelectric focusing of cotyledonous tissue of legumes.Kulturpflanze 32:189-191.
[13] O' Farrell et al.(1977): Higb resolution two-dimensional electrophoresis of basic as well as acidic proteins.Cell l2: 1133-1142.
[14] Romero et al.(1975): Heritable variation in a Polypeptide subunit of the major storage protein of the bean(Phaseolus Vulgaris L.)Pl physiol.56:776-779.
[15] Stegemann(1984): Retrospect on 25 years of cultivar identification by protein Patterns and prospects for the future.in "Biochemical Tests for Cultivar identification" eds.Draper S.R et R.J.Cooke P.20-31.International Seed Testing Association.Zurich.Switzerland.
[16] Yanagi et al.(1984): Variation of protein among eleven varjeries of common bean(Phaseolus Vulgaris) Agric.Biol.Chem.48.2561-2563.

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