华北农学报 ›› 2024, Vol. 39 ›› Issue (1): 8-17. doi: 10.7668/hbnxb.20194481

所属专题: 油料作物 生物技术 热点文章

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

芥菜型油菜AHK家族成员的全基因组鉴定及调控粒质量性状的初步探索

游亮1,2,3, 陈浩3, 肖华贵1, 梁峰豪1, 张超1, 王璐璐1, 唐容1, 杨斌1   

  1. 1 贵州省油料研究所,贵州 贵阳 550006
    2 湖南人文科技学院 农业与生物技术学院,湖南 娄底 417000
    3 湖南农业大学,湖南 长沙 410128
  • 收稿日期:2023-08-30 出版日期:2024-02-28
  • 通讯作者:
    杨斌(1980—),男,四川巴中人,副研究员,博士,主要从事油菜遗传育种研究。
  • 作者简介:

    游亮(1994—),男,湖南新化人,讲师,博士,主要从事油菜重金属胁迫研究。

  • 基金资助:
    黔科合基础-ZK[2023]一般项目(186号); 国家油菜产业技术体系(CARS-12); 贵州省油菜产业技术体系(GZCYTX2018-08); 黔农科院种质资源(202304)

Genome-wide Identification of AHK Family Members in Brassica juncea and Preliminary Exploration of Regulating Seed Weight Traits

YOU Liang1,2,3, CHEN Hao3, XIAO Huagui1, LIANG Fenghao1, ZHANG Chao1, WANG Lulu1, TANG Rong1, YANG Bin1   

  1. 1 Guizhou Institute of Oil Crops,Guiyang 550006,China
    2 College of Agriculture and Biotechnology, Hunan University of Humanities,Science and Technology,Loudi 417000,China
    3 Hunan Agricultural University,Changsha 410128,China
  • Received:2023-08-30 Published:2024-02-28

摘要:

为探究芥菜型油菜AHK家族蛋白的结构特征及其与粒质量性状相关的调控功能,采用生物信息学方法对芥菜型油菜AHK家族成员进行了全基因组鉴定、理化性质、系统发育树、蛋白结构、启动子顺式元件和不同组织表达谱等分析。结果表明,在芥菜型油菜基因组中共鉴定到19条BjAHK蛋白序列,系统发育树分析将BjAHK家族成员分为了4个分支,即G1(BjAHK2~BjAHK4)、G2(BjAHK5)、G3(BjAHK1)和G4(BjCKI1)。功能结构域研究发现,BjAHK家族成员具有6个核心基序(Motif1~Motif6),与HisKA、HATPase_c和REC保守结构域相对应,其中BjAHK1~BjAHK4和BjCKI1蛋白的N端含有较为保守的跨膜结构域,可能与其跨膜作用功能有关。不同组织表达谱研究发现,细胞分裂素受体蛋白基因(BjAHK2~BjAHK4)为泛表达基因,且在根组织中表达量最高。结合已有的芥菜型油菜粒质量性状转录组数据,成功挖掘出4个可能参与粒质量性状调控的基因BjA07.AHK3BjB03.AHK3BjA10.AHK5BjB05.AHK5。其中,BjA07.AHK3BjB03.AHK3可能正向调控种子发育,而BjA10.AHK5BjB05.AHK5则负向调控。结合BjAHK基因家族分析和转录组测序结果,推测BjAHK3BjAHK5基因在调控芥菜型油菜种子发育方面发挥重要功能。

关键词: 芥菜型油菜, AHK家族, 粒质量性状, 表达分析

Abstract:

In order to investigate the structural characteristics of Brassica juncea AHK family proteins and their regulatory functions related to seed weight traits,it employed bioinformatics methods to perform whole-genome identification,physicochemical properties analysis,phylogenetic tree construction,protein structure analysis,promoter cis-elements analysis,and differential tissue expression profiling of the BjAHK family members.The main findings were as follows:a total of 19 BjAHK protein sequences were identified from the B.juncea genome,and phylogenetic analysis classified the BjAHK family members into four branches:G1 (BjAHK2—BjAHK4),G2 (BjAHK5),G3 (BjAHK1),and G4 (BjCKI1).Analysis of functional domains revealed the presence of six conserved motifs (Motif1—Motif6) in BjAHK family members,corresponding to the HisKA,HATPase_c,and REC conserved domains.The N-terminal regions of BjAHK1—BjAHK4 and BjCKI1 proteins exhibited relatively conserved transmembrane domains,which might be associated with their transmembrane function.Differential tissue expression profiling revealed that the genes encoding cytokinin receptor proteins (BjAHK2—BjAHK4) were constitutively expressed genes,with the highest expression levels observed in root tissues.Through integration with existing transcriptomic data on seed weight traits in B.juncea,four candidate genes potentially involved in the regulation of seed weight traits were identified:BjA07.AHK3,BjB03.AHK3,BjA10.AHK5,and BjB05.AHK5.BjA07.AHK3 and BjB03.AHK3 might positively regulate seed development,while BjA10.AHK5 and BjB05.AHK5 might have the opposite effect.Based on the integration of gene family analysis and transcriptome result,it was inferred that BjAHK3 and BjAHK5 genes play an important role in the regulation of seed development in B.juncea.

Key words: Brassica juncea, AHK family, Seed weight traits, Expression analysis

引用本文

游亮, 陈浩, 肖华贵, 梁峰豪, 张超, 王璐璐, 唐容, 杨斌. 芥菜型油菜AHK家族成员的全基因组鉴定及调控粒质量性状的初步探索[J]. 华北农学报, 2024, 39(1): 8-17. doi: 10.7668/hbnxb.20194481.

YOU Liang, CHEN Hao, XIAO Huagui, LIANG Fenghao, ZHANG Chao, WANG Lulu, TANG Rong, YANG Bin. Genome-wide Identification of AHK Family Members in Brassica juncea and Preliminary Exploration of Regulating Seed Weight Traits[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 8-17. doi: 10.7668/hbnxb.20194481.

使用本文

0
    /   /   推荐 /   导出引用

链接本文: http://www.hbnxb.net/CN/10.7668/hbnxb.20194481

               http://www.hbnxb.net/CN/Y2024/V39/I1/8