牦牛<i>KIF2A</i>基因克隆及其在卵泡与卵母细胞发育过程的表达规律

doi:10.7668/hbnxb.20193224

摘要/Abstract

摘要：

旨在克隆牦牛驱动蛋白家族成员2A基因 (KIF2A),探究其在牦牛不同组织中的表达模式,以及在卵泡发育和卵母细胞成熟过程中的表达规律,以3~4岁健康、未妊娠母牦牛心脏、肝脏、脾脏、肺脏、肾脏、胃、子宫和卵巢组织(n=5)为研究材料,使用RT-PCR技术获得KIF2A基因的编码区序列(CDS),运用MEGA 7.0、SWISS-MODEL等软件分析其生物学特性。使用实时荧光定量PCR(Quantitative Real-time PCR,RT-qPCR)检测KIF2A在牦牛各组织中的表达水平;采用免疫组织化学技术分析KIF2A在牦牛不同直径卵泡中的表达模式及定位。将卵泡按照直径的大小分为3组:小(1.0~2.9 mm)、中(3.0~5.9 mm)、大(6.0~9.0 mm)卵泡;RT-qPCR检测KIF2A基因在不同发育时期卵泡中颗粒细胞、卵母细胞减数分裂3个关键时期的表达特性。结果显示,KIF2A基因序列长为1 964 bp,其中CDS为1 530 bp,编码509个氨基酸,KIF2A蛋白总体带负电,属于疏水性蛋白。KIF2A基因在牦牛各组织中广泛表达,牦牛KIF2A蛋白主要定位于颗粒细胞,且伴随着卵泡的生长发育其在不同直径大小卵泡颗粒细胞中mRNA表达量呈上升趋势。在牦牛卵母细胞成熟过程中,KIF2A基因的表达具有时序特征,其mRNA的表达量呈下降趋势,GⅤ期显著高于MⅠ期和MⅡ期。综上所述,KIF2A可能参与调控牦牛卵泡发育和卵母细胞的成熟过程。

关键词: 牦牛, KIF2A基因, 序列特征, 组织表达, 卵母细胞

Abstract:

The aim of this study was to clone the kinesin family member 2A gene(KIF2A),and explore the expression pattern of KIF2A gene in different tissues of yak,as well as the spatially and temporally expressed during the follicular development and oocyte meiotic maturation.The heart,liver,spleen,lung,kidney stomach,uterus and ovary tissues(n=5)were collected from 3—4 years old healthy female yak after slaughter.Yak KIF2A gene was amplified by RT-PCR with yak ovarian cDNA as template,and its protein were analyzed by bioinformatics software such as MAGA 7.0 and SWISS-MODEL etc.Quantitative Real-time PCR(RT-qPCR)was used to detect the expression level of KIF2A in yak various tissues and immunohistochemistry was used to analyze the expression and localization of KIF2A in follicles with different diameters.According to the diameter,the follicle were divided into three groups: small(diameter 1.0—2.9 mm),medium(diameter 3.0—5.9 mm),and large(diameter 6.0—9.0 mm)follicle.The expression characteristics of KIF2A gene in granulosa cells and oocyte meiosis in follicles were analyzed by RT-qPCR.The results showed that sequence length of KIF2A gene was 1 964 bp,and the CDS was 1 530 bp,which encoding 509 amino acids.KIF2A protein was negatively charged and belonged to hydrophobic protein.KIF2A gene was relatively conservative and widely expressed in various tissues of yaks, and KIF2A protein was mainly located in granulosa cells, its mRNA expression in follicular granulosa cells with different diameter and size increased with the growth and development of follicles. During the meiotic maturation of yak oocytes,the expression of KIF2A showed a sequential feature,and the G Ⅴ phase was significantly higher than that of M Ⅰ and M Ⅱ phase.The results showed that KIF2A might be involved in the regulation of follicular and oocyte maturation development in yaks,and the results of this study provided basic data for the functional study of KIF2A gene in yak follicular development.

Key words: Yak, KIF2A genes, Sequence characteristics, Tissue expression, Oocyte

杨满珍, 闵星宇, 杨璐瑜, 于海玲, 胡宇磊, 朱艳锦, 潘帮婷, 李键, 熊显荣. 牦牛KIF2A基因克隆及其在卵泡与卵母细胞发育过程的表达规律[J]. 华北农学报, 2022, 37(6): 219-227. doi: 10.7668/hbnxb.20193224.

YANG Manzhen, MIN Xingyu, YANG Luyu, YU Hailing, HU Yulei, ZHU Yanjin, PAN Bangting, LI Jian, XIONG Xianrong. Cloning and Expression of KIF2A Gene During Follicular and Oocyte Development in Yak[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 219-227. doi: 10.7668/hbnxb.20193224.

http://www.hbnxb.net/CN/Y2022/V37/I6/219

图/表 9

表1 PCR引物序列及产物信息

Tab.1 PCR primer sequences and products information

基因 Gene	引物(5'—3') Sequence of primer(5'—3')	产物长度/bp Product size	退火温度/℃ Tm	用途 Utilization
KIF2A	F:TTTGCTTATGGGCAGACT	1 964	59	克隆
	R:ACATTTGACCTCCCGTTC
KIF2A	F:TCCCACCACTTGAACCTG	151	60	RT-qPCR
	R:AATGCTAAAGAAGCCGAACT
GAPDH	F:TGCGTGCTGAGTAGTTGGTG	290	59	RT-qPCR
	R:TCTTCTGGGTGGCAGTGATGG

表2 生物信息学分析内容和工具

Tab.2 Bioinformatics analysis contentsand tools

分析内容 Analysis contents	分析工具 Analysis tools
开放阅读框的预测及氨基酸序列的翻译	https://www.ncbi.nlm.nih.gov/orffinder/
Prediction of ORF finder and translation of amino acid sequence
序列比对Sequence alignment	https://blast.ncbi.nlm.nih.gov/Blast.cgi
蛋白质理化性质分析Analysis of physicochemical properties of proteins	https://web.expasy.org/protparam/
蛋白质亲疏水性分析Hydrophilic and hydrophobic analysis of proteins	https://web.expasy.org/protscale/
相互作用蛋白预测Interaction protein prediction	https://cn.string-db.org/cgi/networki
蛋白质功能结构域预测Prediction of protein functional domain	https://myhits.sib.swiss/cgi-bin/motif_scan
蛋白质二级结构的预测Prediction of protein secondary structure	https://npsa-prabi.ibcp.fr/cgi-bin/
蛋白质三级结构的预测Prediction of tertiary structure of proteins	https://swissmodel.expasy.org/interactive
物种间氨基酸序列比对Amino acid sequence alignment between species	DNAMAN
构建进化树Construction of evolutionary trees	MEGA 6.0

图1 牦牛KIF2A基因扩增、序列比对 A:M.DL2000 DNA Marker,1.KIF2A基因;B.牦牛KIF2A基因预测序列及推测的氨基酸序列;C.测定序列与黄牛KIF2A编码区序列差异。

Fig.1 Amplification,and sequence comparison of yak KIF2A gene A: M.DL2000 DNA Marker,1.KIF2A gene;B. Predicted sequence of KIF2A gene and predicted amino acid sequence of yak;C. The difference between the determination sequence and the KIF2A coding region sequence of cattle.

图2 牦牛KIF2A基因生物信息学 A.牦牛KIF2A蛋白亲疏水性分析;B.牦牛KIF2A蛋白磷酸化位点分析;C.牦牛KIF2A蛋白互作蛋白预测。

Fig.2 Bioinformatics of yak KIF2A gene A.Hydrophilic and hydrophobic analysis of yak KIF2A protein;B.Phosphorylation site analysis of yak KIF2A protein;C.Prediction of yak KIF2A protein interaction protein.

图3 牦牛KIF2A蛋白功能结构域、二级结构和三级结构 A.牦牛KIF2A蛋白功能结构域分析;B.牦牛KIF2A二级结构;C.牦牛KIF2A蛋白三级结构。

Fig.3 Functional domain,secondary structure and tertiary structure of yak KIF2A protein A.Functional domain analysis of yak KIF2A protein;B.The secondary structure of yak KIF2A protein;C.The tertiary structure of yak KIF2A protein.

图4 牦牛KIF2A基因同源性分析、进化树图谱 A.不同物种间KIF2A同源性分析;B.牦牛KIF2A系统发育树。

Fig.4 Homology analysis and phylogenetic tree of yak KIF2A gene A.Homology analysis of KIF2A among different species;B.Phylogenetic tree of yak KIF2A.

图5 KIF2A基因在不同中的组织表达水平不同小写字母表示差异显著(P<0.05)。图7同。

Fig.5 Relative expression levels of KIF2A gene in different tissues Different lowercase letters indicate significant difference (P<0.05).The same as Fig.7.

图6 牦牛卵巢中KIF2A的表达定位 A.牦牛卵巢概览图;B.有腔卵泡;C.有腔卵泡局部放大:GC.壁层颗粒细胞,TC.膜细胞。

Fig.6 Expression and location of KIF2A in yak ovary A.General picture of yak ovary;B.Antral follicle,C.Local enlargement of antral follicle: GC.Granular cell,TC.Theca cells.

图7 牦牛不同发育阶段卵泡颗粒细胞、不同时期卵母细胞KIF2A的相对表达水平 A.小、中、大卵泡颗粒细胞KIF2A的相对表达水平;B.不同时期卵母细胞KIF2A的相对表达水平。

Fig.7 Relative expression levels of KIF2A in follicular granulosa cells and oocytes of yaks at different developmental stages A.Relative expression levels of KIF2A in small,medium and large follicular granulosa cells; B.Relative expression levels of KIF2A in oocytes at different stages.

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牦牛KIF2A基因克隆及其在卵泡与卵母细胞发育过程的表达规律

Cloning and Expression of KIF2A Gene During Follicular and Oocyte Development in Yak

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Cloning and Expression of KIF2A Gene During Follicular and Oocyte Development in Yak

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