粗糙脉孢菌NC-3菌株对水稻秸秆降解的影响

doi:10.7668/hbnxb.20193067

摘要/Abstract

摘要：

为了明确粗糙脉孢菌NC-3菌株在水稻秸秆还田上的应用潜力,采用室内培养和盆栽试验相结合的方法,分析NC-3菌株在水稻秸秆上的定殖,对水稻秸秆降解,纤维素、半纤维素、木质素、总酚酸含量以及油菜发芽率和成苗率的影响。结果表明,NC-3菌株能在灭菌的水稻秸秆上迅速定殖(72 h长满菌丝和孢子)。相比无菌水,培养7,14,21 d,NC-3菌株处理的水稻秸秆降解率分别提高2.3,7.3,3.2百分点;秸秆纤维素、木质素以及总酚酸含量分别下降2.0,10.7,10.4百分点,0.7,0.9,1.3百分点和7.6%,6.9%,6.4%。NC-3菌株对水稻秸秆、纤维素、木质素和总酚酸的降解作用主要发生在培养的前14 d(0~14 d),对半纤维素的降解作用在培养14 d后逐渐增强。添加水稻秸秆会显著降低油菜发芽率和成苗率,且随秸秆用量的增加抑制作用增强,但接种NC-3菌株能显著提高油菜发芽率和成苗率(相比无菌水,分别提高3.3,9.7百分点)。总之,接种脉孢菌NC-3菌株能有效加快水稻秸秆降解和酚酸类物质转化,可有效提高油菜发芽率和成苗率。

关键词: 粗糙脉孢菌, NC-3菌株, 水稻秸秆, 降解, 油菜幼苗

Abstract:

To clarify the application potential of Neurospora crassa NC-3 strain on rice straw returning,a combination of indoor simulation and pot experiment were conducted to investigate the effects of NC-3 strain on degradation,cellulose,hemicellulosic,lignin,total phenolic acid content of rice straw,germination rate and seedling rate of rapeseed.The results showed that NC-3 strain could rapidly colonize on sterilized rice straw(full of mycelia and spores at 72 h).Compared with the sterile water,the degradation rate of rice straw in NC-3 strain was increased by 2.3,7.3 and 3.2 percentage points at 7,14 and 21 days,respectively.The contents of cellulose,lignin and total phenolic acid decreased with NC-3 strain by 2.0,10.7,10.4 percentage points,0.7,0.9,1.3 percentage points and 7.6%,6.9%,6.4%,respectively.The degradation effect of NC-3 strain on cellulose,lignin and total phenolic acid of rice straw mainly occurred in the first two weeks(0—14 days)of straw,and the degradation effect on hemicellulose gradually increased after 14 days of culture.Addition of rice straw significantly reduced the germination rate and seedling rate of rapeseed,and with the increase of straw dosage,the inhibition effect was enhanced,but NC-3 strain could significantly increase the germination rate and seedling rate of rapeseed(3.3 and 9.7 percentage points higher than sterile water in petri dish test,respectively).In summary,NC-3 strain could effectively accelerate the degradation of rice straw and the transformation of phenolic acids,and effectively improve the germination rate and seedling formation rate of rapeseed.

Key words: Neurospora crassa, NC-3 strain, Rice straw, Degradation, Rapeseed seedling

李银水, 余常兵, 戴志刚, 顾炽明, 李越, 廖星, 谢立华, 胡小加, 秦璐. 粗糙脉孢菌NC-3菌株对水稻秸秆降解的影响[J]. 华北农学报, 2023, 38(1): 154-161. doi: 10.7668/hbnxb.20193067.

LI Yinshui, YU Changbing, DAI Zhigang, GU Chiming, LI Yue, LIAO Xing, XIE Lihua, HU Xiaojia, QIN Lu. Effects of Neurospora crassa NC-3 Strain on Degradation of Rice Straw[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 154-161. doi: 10.7668/hbnxb.20193067.

http://www.hbnxb.net/CN/Y2023/V38/I1/154

图/表 8

图1 脉孢菌NC-3菌株在水稻秸秆上的定殖

Fig.1 Colonization of Neurospora crassa (NC-3)on rice straw A、C.CK 24,72 h;B、D.NC-3 24,72 h。Bars=1.0 mm。

图2 脉孢菌NC-3菌株对水稻秸秆降解率的影响不同字母分别表示在0.05水平差异显著。图3—8同。

Fig.2 Effect of Neurospora crassa (NC-3) on degradation rate of rice straw Different letters are significantly different at 0.05 level.The same as Fig.3—8.

图3 脉孢菌NC-3菌株对水稻秸秆纤维素含量的影响

Fig.3 Effect of Neurospora crassa (NC-3) on cellulose content in rice straw

图4 脉孢菌NC-3菌株对水稻秸秆半纤维素含量的影响

Fig.4 Effect of Neurospora crassa (NC-3) on hemicellulose content in rice straw

图5 脉孢菌NC-3菌株对水稻秸秆木质素含量的影响

Fig.5 Effect of Neurospora crassa (NC-3) on lignin content in rice straw

图6 脉孢菌NC-3菌株对水稻秸秆总酚酸含量的影响

Fig.6 Effect of Neurospora crassa (NC-3) on total phenolic acid content in rice straw

图7 脉孢菌NC-3菌株对油菜发芽率和成苗率的影响(培养皿)

Fig.7 Effect of Neurospora crassa (NC-3) on germination rate and seedling rate of rapeseed

图8 脉孢菌NC-3菌株对油菜发芽率和成苗率的影响(盆栽)

Fig.8 Effect of Neurospora crassa (NC-3) on germination rate and seeding rate of rapeseed

参考文献 34

[1]	王昆昆, 刘秋霞, 朱芸, 李小坤, 任涛, 鲁剑巍, 丛日环. 稻草覆盖还田对直播冬油菜生长及养分积累的影响[J]. 植物营养与肥料学报, 2019, 25(6):1047-1055.doi:10.11674/zwyf.18215. doi: 10.11674/zwyf.18215
	Wang K K, Liu Q X, Zhu Y, Li X K, Ren T, Lu J W, Cong R H. Effects of straw mulching on growth and nutrients accumulation of direct-sown winter oilseed rape[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(6):1047-1055.
[2]	梅沛沛, 余常兵, 吴言凤, 李银水, 胡喜巧, 黄玲. 稻秸还田方式对酸性和碱性土壤养分含量、微生物数量及油菜生长的影响[J]. 土壤通报, 2020, 51(2):365-371.doi:10.19336/j.cnki.trtb.2020.02.14. doi: 10.19336/j.cnki.trtb.2020.02.14
	Mei P P, Yu C B, Wu Y F, Li Y S, Hu X Q, Huang L. Effects of rice straw returning on soil properties,microbial number and rape growth in acid and alkaline soils[J]. Chinese Journal of Soil Science, 2020, 51(2):365-371. doi: 10.1046/j.1365-2389.2000.00312.x URL
[3]	李银水, 余常兵, 戴志刚, 顾炽明, 秦璐, 沈欣杰, 胡小加, 谢立华, 廖星. 稻秆还田方式对油菜产量及养分效率的影响[J]. 华北农学报, 2021, 36(1):177-186.doi:10.7668/hbnxb.20191303. doi: 10.7668/hbnxb.20191303
	Li Y S, Yu C B, Dai Z G, Gu C M, Qin L, Shen X J, Hu X J, Xie L H, Liao X. Effects of rice straw returning patterns on rapeseed yields and nutrient efficiency[J]. Acta Agriculturae Boreali-Sinica, 2021, 36(1):177-186. doi: 10.7668/hbnxb.20191303
[4]	苏伟, 鲁剑巍, 周广生, 李小坤, 李云春, 刘晓伟. 稻草还田对油菜生长、土壤温度及湿度的影响[J]. 植物营养与肥料学报, 2011, 17(2):366-373.doi:10.11674/zwyf.2011.0287. doi: 10.11674/zwyf.2011.0287
	Su W, Lu J W, Zhou G S, Li X K, Li Y C, Liu X W. Influence of straw-returning on rapeseed(Brassica napus L.)growth,soil temperature and moisture[J]. Journal of Plant Nutrition and Fertilizers Science, 2011, 17(2):366-373.
[5]	Cao Y F, Zhang H, Liu K, Dai Y C, Lü J L. Organic acids variation in plant residues and soils among agricultural treatments[J]. Agronomy, Journal, 2015, 107(6):2171-2180.doi:10.2134/agronj15.0137. doi: 10.2134/agronj15.0137 URL
[6]	李晶, 赵先龙, 乔天长, 顾万荣, 焦健, 李改玲, 罗宁, 魏湜. 秸秆腐解液对玉米幼苗的生理效应及酚酸类化感成分的检测[J]. 核农学报, 2015, 29(9):1799-1805.doi:10.11869/j.issn.100-8551.2015.09.1799. doi: 10.11869/j.issn.100-8551.2015.09.1799
	Li J, Zhao X L, Qiao T C, Gu W R, Jiao J, Li G L, Luo N, Wei S. Physiological effects of maize stalk decomposition liquid on seedlings and detection of phenolic allelochemical ingredients[J]. Journal of Nuclear Agricultural Sciences, 2015, 29(9):1799-1805.
[7]	刘瑞显, 张国伟, 杨长琴, 张雷, 倪万潮. 小麦秸秆浸提液和腐解液对棉花种子发芽及幼苗生长的化感效应[J]. 棉花学报, 2016, 28(4):375-383.doi:10.11963/issn.1002-7807.201604009. doi: 10.11963/issn.1002-7807.201604009
	Liu R X, Zhang G W, Yang C Q, Zhang L, Ni W C. Allelopathic effects of wheat straw extract and decomposition liquid on cotton seed germination and seedling growth[J]. Cotton Science, 2016, 28(4):375-383.
[8]	Sun J P, Tian C G, Diamond S, Glass N L. Deciphering transcriptional regulatory mechanisms associated with hemicellulose degradation in Neurospora crassa[J]. Eukaryotic Cell, 2012, 11(4):482-493.doi:10.1128/EC.05327-11. doi: 10.1128/EC.05327-11 URL
[9]	林良才, 李金根, 王邦, 裴雪, 田朝光. 粗糙脉孢菌木质纤维素降解利用研究进展[J]. 生物加工过程, 2014, 12(1):28-36.doi:10.3969/j.issn.1672-3678.2014.01.006. doi: 10.3969/j.issn.1672-3678.2014.01.006
	Lin L C, Li J G, Wang B, Pei X, Tian C G. Progress of lignocellulose degradation and utilization on model filamentous fungus Neurospora crassa[J]. Chinese Journal of Bioprocess Engineering, 2014, 12(1):28-36.
[10]	谢立华, 淡育红, 胡小加, 余常兵, 李银水, 秦璐, 张银波, 胡磊, 廖星. 促进作物秸秆和菌核腐解的复合生物制剂应用效果[J]. 中国油料作物学报, 2015, 37(3):372-376.doi:10.7505/j.issn.1007-9084.2015.03.018. doi: 10.7505/j.issn.1007-9084.2015.03.018
	Xie L H, Dan Y H, Hu X J, Yu C B, Li Y S, Qin L, Zhang Y B, Hu L, Liao X. Application effect of compound microbial agents promoting crop straw and Sclerotia decomposition[J]. Chinese Journal of Oil Crop Sciences, 2015, 37(3):372-376.
[11]	叶永华, 张双, 游立群, 江颖倩, 许文, 叶淼, 徐伟. 福林酚法测定闽产盐肤木根茎中总酚酸的含量[J]. 福建中医药, 2018, 49(2):66-67.doi:10.13260/j.cnki.jfjtcm.011602. doi: 10.13260/j.cnki.jfjtcm.011602
	Ye Y H, Zhang S, You L Q, Jiang Y Q, Xu W, Ye M, Xu W. Determination of total phenolic acid in rhizome of Rhus chinensis Mill.by folinol method[J]. Fujian Journal of Traditional Chinese Medicine, 2018, 49(2):66-67.
[12]	王玉万, 徐文玉. 木质纤维素固体基质发酵物中半纤维素、纤维素和木素的定量分析程序[J]. 微生物学通报, 1987, 14(2):81-84.doi:10.13344/j.microbiol.china.1987.02.012. doi: 10.13344/j.microbiol.china.1987.02.012
	Wang Y W, Xu W Y. Quantitative analysis procedure for hemicellulose,cellulose and lignin in lignocellulosic solid substrate fermentation[J]. Microbiology, 1987, 14(2):81-84.
[13]	孙海涛, 陈志玲. 粗糙脉孢菌驱动蛋白-1在菌丝极性生长中的功能[J]. 首都师范大学学报(自然科学版), 2022, 43(2):49-55.doi:10.19789/j.1004-9398.2022.02.008. doi: 10.19789/j.1004-9398.2022.02.008
	Sun H T, Chen Z L. Functional analysis of kinesin-1 in hyphal polarized growth of Neurospora crassa[J]. Journal of Capital Normal University (Natural Science Edition), 2022, 43(2):49-55.
[14]	戴志刚, 鲁剑巍, 李小坤, 鲁明星, 杨文兵, 高祥照. 不同作物还田秸秆的养分释放特征试验[J]. 农业工程学报, 2010, 26(6):272-276.doi:10.3969/j.issn.1002-6819.2010.06.047. doi: 10.3969/j.issn.1002-6819.2010.06.047
	Dai Z G, Lu J W, Li X K, Lu M X, Yang W B, Gao X Z. Nutrient release characteristic of different crop straws manure[J]. Transactions of the Chinese Society of Agricultural Engineering, 2010, 26(6):272-276.
[15]	徐健程, 王晓维, 朱晓芳, 邓晓东, 杨文亭. 不同绿肥种植模式下玉米秸秆腐解特征研究[J]. 植物营养与肥料学报, 2016, 22(1):48-58.doi:10.11674/zwyf.14507. doi: 10.11674/zwyf.14507
	Xu J C, Wang X W, Zhu X F, Deng X D, Yang W T. Study on decomposition of maize straw under different green manure cropping patterns[J]. Journal of Plant Nutrition and Fertilizers, 2016, 22(1):48-58.
[16]	王西亚, 吕继龙, 何萍, 范分良, 仇少君, 徐新朋, 赵士诚. 玉米秸秆分解过程中细菌群落组成演化特征[J]. 植物营养与肥料学报, 2021, 27(1):45-53.doi:10.11674/zwyf.20242. doi: 10.11674/zwyf.20242
	Wang X Y, Lü J L, He P, Fan F L, Qiu S J, Xu X P, Zhao S C. Succession in bacterial community during maize straw decomposition[J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(1):45-53.
[17]	Lee C G, Watanabe T, Sato Y, Murase J, Asakawa S, Kimura M. Bacterial populations assimilating carbon from ¹³C-labeled plant residue in soil:Analysis by a DNA-SIP approach[J]. Soil Biology and Biochemistry, 2011, 43(4):814-822.doi:10.1016/j.soilbio.2010.12.016. doi: 10.1016/j.soilbio.2010.12.016 URL
[18]	Fan F L, Yin C, Tang Y J, Li Z J, Song A L, Wakelin S A, Zou J, Liang Y C. Probing potential microbial coupling of carbon and nitrogen cycling during decomposition of maize residue by ¹³C-DNA-SIP[J]. Soil Biology and Biochemistry, 2014, 70:12-21.doi:10.1016/j.soilbio.2013.12.002. doi: 10.1016/j.soilbio.2013.12.002 URL
[19]	李培培, 张冬冬, 王小娟, 王小芬, 崔宗均. 促分解菌剂对还田玉米秸秆的分解效果及土壤微生物的影响[J]. 生态学报, 2012, 32(9):2847-2854.doi:10.5846/stxb201103150321. doi: 10.5846/stxb201103150321
	Li P P, Zhang D D, Wang X J, Wang X F, Cui Z J. Effects of microbial inoculants on soil microbial diversity and degrading process of corn straw returned to field[J]. Acta Ecologica Sinica, 2012, 32(9):2847-2854. doi: 10.5846/stxb URL
[20]	王文山, 王维敏, 张镜清, 蔡典雄, 张美珠. 农作物残体在北京农田土壤中的分解[J]. 土壤通报, 1989, 20(3):113-115,112.doi:10.19336/j.cnki.trtb.1989.03.005. doi: 10.19336/j.cnki.trtb.1989.03.005
	Wang W S, Wang W M, Zhang J Q, Cai D X, Zhang M Z. Decomposition of crop residues in Beijing farmland soil[J]. Chinese Journal of Soil Science, 1989, 20(3):113-115,112.
[21]	Fierer N, Bradford M A, Jackson R B. Toward an ecological classification of soil bacteria[J]. Ecology, 2007, 88(6):1354-1364.doi:10.1890/05-1839. doi: 10.1890/05-1839 pmid: 17601128
[22]	苏朋, 傅昱, 何艳, 徐建明, 吴建军, 吴良欢. 控制条件下水肥耦合对黄泥田还田秸秆腐解及土壤碳转化的影响[J]. 植物营养与肥料学报, 2015, 21(1):1-11.doi:10.11674/zwyf.2015.0101. doi: 10.11674/zwyf.2015.0101
	Su P, Fu Y, He Y, Xu J M, Wu J J, Wu L H. Effect of soil moisture and nitrogen fertilizer on the decomposition of straw returned to field and the transformation of carbon under controlled conditions[J]. Journal of Plant Nutrition and Fertilizers, 2015, 21(1):1-11.
[23]	陈晏, 戴传超, 王兴祥, 张波, 鞠群. 施加内生真菌拟茎点霉(Phomopsis sp.)对茅苍术凋落物降解及土壤降解酶活性的影响[J]. 土壤学报, 2010, 47(3):537-544.doi:10.11766/trxb200904190321. doi: 10.11766/trxb200904190321
	Chen Y, Dai C C, Wang X X, Zhang B, Ju Q. Effects of endophytic fungus(Phomopsis sp.)on decomposition of plant(Atractylodes lancea(thunb)dc)litters and activity of degrading enzymes in soil[J]. Acta Pedologica Sinica, 2010, 47(3):537-544.
[24]	牛文娟. 主要农作物秸秆组成成分和能源利用潜力[D]. 北京: 中国农业大学, 2015.
	Niu W J. Physicochemical composition and energy potential of main crop straw and stalk[D]. Beijing: China Agricultural University, 2015.
[25]	Yan C, Yan S S, Jia T Y, Dong S K, Ma C M, Gong Z P. Decomposition characteristics of rice straw returned to the soil in Northeast China[J]. Nutrient Cycling in Agroecosystems, 2019, 114(3):211-224.doi:10.1007/s10705-019-09999-8. doi: 10.1007/s10705-019-09999-8
[26]	王博伟, 闫雪晴, 何贤, 邹春阳, 吴文娟. 纤维素可及性对木质纤维酶水解影响的研究进展[J]. 纤维素科学与技术, 2020, 28(1):61-68.doi:10.16561/j.cnki.xws.2020.01.01. doi: 10.16561/j.cnki.xws.2020.01.01
	Wang B W, Yan X Q, He X, Zou C Y, Wu W J. Progress of the effects of cellulose accessibility on enzymatic hydrolysis of lignocellulose[J]. Journal of Cellulose Science and Technology, 2020, 28(1):61-68.
[27]	Jia J R, Chen H Y, Wu B G, Ni Z. Cations optimization for protein enrichment in rice straw by mixed cultures of Neurospora crassa 14-8 and Candida utilis using response surface methodology[J]. Applied Biochemistry and Biotechnology, 2017, 182(2):804-817.doi:10.1007/s12010-016-2363-6. doi: 10.1007/s12010-016-2363-6 URL
[28]	Mach R L, Zeilinger S. Regulation of gene expression in industrial fungi:Trichoderma[J]. Applied Microbiology and Biotechnology, 2003, 60(5):515-522.doi:10.1007/s00253-002-1162-x. doi: 10.1007/s00253-002-1162-x pmid: 12536250
[29]	石祖梁, 邵宇航, 王飞, 王久臣, 孙仁华, 宋成军, 李想. 我国秸秆综合利用面临形势与对策研究[J]. 中国农业资源与区划, 2018, 39(10):30-36.doi:10.7621/cjarrp.1005-9121.20181005. doi: 10.7621/cjarrp.1005-9121.20181005
	Shi Z L, Shao Y H, Wang F, Wang J C, Sun R H, Song C J, Li X. Study on the situation and countermeasures of straw comprehensive utilization in China[J]. Chinese Journal of Agricultural Resources and Regional Planning, 2018, 39(10):30-36.
[30]	于建光, 常志州, 王宁, 石祖梁, 卢信, 张传辉. 高效液相色谱法测定秸秆浸提液或腐解液中12种酚酸[J]. 农业环境科学学报, 2016, 35(11):2231-2236.doi:10.11654/jaes.2016-0591. doi: 10.11654/jaes.2016-0591
	Yu J G, Chang Z Z, Wang N, Shi Z L, Lu X, Zhang C H. Determination of 12 phenolic acids in straw extract or straw decomposed liquid through high performance liquid chromatography[J]. Journal of Agro-Environment Science, 2016, 35(11):2231-2236.
[31]	顾元, 常志州, 于建光, 宗良纲. 外源酚酸对水稻种子和幼苗的化感效应[J]. 江苏农业学报, 2013, 29(2):240-246.doi:10.3969/j.issn.1000-4440.2013.02.002. doi: 10.3969/j.issn.1000-4440.2013.02.002
	Gu Y, Chang Z Z, Yu J G, Zong L G. Allelopathic effects of exogenous phenolic acids composted by wheat straw on seed germination and seedling growth of rice[J]. Jiangsu Journal of Agricultural Sciences, 2013, 29(2):240-246.
[32]	张国伟, 刘瑞显, 杨长琴, 杨富强. 棉花秸秆浸提液对小麦种子萌发及幼苗生长的化感效应[J]. 麦类作物学报, 2015, 35(4):555-562.doi:10.7606/j.issn.1009-1041.2015.04.17. doi: 10.7606/j.issn.1009-1041.2015.04.17
	Zhang G W, Liu R X, Yang C Q, Yang F Q. Allelopathic effects of cotton straw extract on seed germination and seedling growth of wheat[J]. Journal of Triticeae Crops, 2015, 35(4):555-562.
[33]	Chen Y, Wang H W, Li L, Dai C C. The potential application of the endophyte Phomopsis liquidambari to the ecological remediation of long-term cropping soil[J]. Applied Soil Ecology, 2013, 67:20-26.doi:10.1016/j.apsoil.2013.02.004. doi: 10.1016/j.apsoil.2013.02.004 URL
[34]	谢星光, 戴传超, 苏春沦, 周佳宇, 王宏伟, 王兴祥. 内生真菌对花生残茬腐解及土壤酚酸含量的影响[J]. 生态学报, 2015, 35(11):3836-3845.doi:10.5846/stxb201308132074. doi: 10.5846/stxb201308132074
	Xie X G, Dai C C, Su C L, Zhou J Y, Wang H W, Wang X X. Effects of an endophytic fungus on decay of peanut residues and phenolic acid concentrations in soil[J]. Acta Ecologica Sinica, 2015, 35(11):3836-3845.

选择文件类型/文献管理软件名称

选择包含的内容