摘要: 呋喃丹是一种高残留、高毒性、难降解的有机杀虫剂,目前在我国的广泛使用,常造成人畜中毒和环境污染。采用富集分离的方法从常年使用呋喃丹的土壤中分离得到一株呋喃丹降解菌CF-14,根据其生理生化特征及16 S rDNA分子生物学鉴定,该菌属于无色杆菌属。CF-14降解呋喃丹的初选培养条件为温度30℃,pH 7.0,接种量为10%,转速达到200 r/min时,3 d对浓度为100 mg/L的呋喃丹降解率为95%。
关键词:
呋喃丹,
降解,
无色杆菌属
Abstract: Carbofuran is a kind of high residue,high toxicity,difficult degradation organic pesticide,which is widely used in China and often caused human and animal poisoning and environmental pollution.We isolate one strain carbofuran-degrading bacterium from the soil of perennial using carbofuran by enrichment separation method.It was identified as Achromobacter sp according to its biochemical characteristics,morphology and 16 S rDNA molecular biology appraisal.The results of the degrading tests showed that the preliminary culture conditions were temperature 30℃,pH 7.0 and inoculation quantity 10%.It degraded carbofuran in the rate of 95% for 3 days.
Key words:
Carbofuran,
Degradation,
Achromobacter
中图分类号:
荆新堂, 李勤凡, 张丽慧, 孔祥雅. 呋喃丹降解菌CF-14的筛选及降解条件研究[J]. 华北农学报, 2011, 26(S1): 229-232. doi: 10.7668/hbnxb.2011.S1.046.
JING Xin-tang, LI Qin-fan, ZHANG Li-hui, KONG Xiang-ya. The Screening of a Carbofuran-degrading Bacteria Strain Achromobacter sp. CF-14 and Its Degradation Conditions Research[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 229-232. doi: 10.7668/hbnxb.2011.S1.046.