玉米ERD基因的表达模式及<i>ZmERD6</i>基因的抗旱性分析

doi:10.7668/hbnxb.20190812

摘要/Abstract

摘要： 为了进一步了解玉米ZmERD基因，基于旱胁迫转录组数据，筛选出5个ZmERD基因。将这些基因编码的蛋白质与拟南芥16个ERD蛋白进行进化分析发现， GRMZM2G181206和GRMZM2G128641基因编码的蛋白质分别与AT4G02900和AT1G32090基因编码的蛋白质同源性较高。时空表达分析发现， GRMZM2G109201、GRMZM2G181206和GRMZM2G12864基因属于组成型表达， GRMZM2G134192基因属于特异型表达，只在花药中高表达。属于ERD6亚家族的GRMZM2G109201基因在旱敏感型（B73）和抗旱型（郑58）自交系中均表达，且郑58中表达量显著高于B73；随着旱胁迫时间的延长， GRMZM2G109201基因在B73中表达量差异不显著，但在郑58中表达量升高幅度达到显著水平。亚细胞定位显示， GRMZM2G109201基因编码的ZmERD6蛋白定位于质膜。推测GRMZM2G109201基因参与旱胁迫，且被正向诱导。

关键词: 玉米, 早期脱水反应基因, 进化, 表达模式, 抗旱性, 亚细胞定位

Abstract: The early responsive to dehydration(ERD)gene is a kind of functional gene that can respond quickly when plants encounter water stress,and plays an important role in slowing the stress of plants suffering from water stress. This type of gene is excavated and new drought-resistant varieties can be cultivated through molecular-assisted breeding. Therefore,based on previous studies,in order to further understand the ZmERD genes,this study screened five ZmERD genes based on drought stress transcriptome data. The proteins encoded by these genes were analyzed with AtERD protein,and the proteins encoded by the GRMZM2G181206 and GRMZM2G128641 genes were found to have higher homology with the proteins encoded by AT4G02900 and the AT1G32090 protein,respectively. The spatiotemporal expression analysis revealed that the GRMZM2G109201,GRMZM2G181206 and GRMZM2G12864 genes were constitutively expressed,and the GRMZM2G134192 gene was a specific expression,which was significantly highly expressed only in anthers. The GRMZM2G109201 gene belonging to the ERD6 subfamily was expressed in both drought-sensitive(B73)and drought-resistant(Zheng 58)inbred lines,and the expression level in Zheng 58 was significantly higher than that in B73. With the prolongation of drought stress,the expression level of GRMZM2G109201 gene was not significantly different in B73, but the expression level in Zheng 58 increased significantly. Subcellular localization revealed that the ZmERD6 protein encoded by the GRMZM2G109201 gene was located in the plasma membrane. We speculated that the GRMZM2G109201 gene was involved in drought stress and was positively induced.

Key words: Maize, ERD gene, Evolution, Expression pattern, Drought resistance, Subcellular localization

中图分类号:

S513

刘松涛, 曹雯梅, 郑贝贝, 赵威. 玉米ERD基因的表达模式及ZmERD6基因的抗旱性分析[J]. 华北农学报, 2020, 35(1): 51-56. doi: 10.7668/hbnxb.20190812.

LIU Songtao, CAO Wenmei, ZHENG Beibei, ZHAO Wei. Expression Pattern of ERD Genes in Maize and Drought Resistance Analysis of ZmERD6 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 51-56. doi: 10.7668/hbnxb.20190812.

http://www.hbnxb.net/CN/Y2020/V35/I1/51

参考文献

[1] 吴子恺.玉米抗旱育种[J].玉米科学,1994,2(1):6-9.
Wu Z K.Corn breeding for drought resistance[J].Maize Science,1994,2(1):6-9.
[2] Bray E A. Plant responses to water deficit[J].Trends in Plant Science,1997,2(2):48-54.doi:10.1016/S1360-1385(97)82562-9.
[3] 却志群,於紫蕾,沈春修.水稻膜联蛋白基因 OsAnn8 干旱和低温条件下表达模式以及CRISPR/Cas9定点编辑[J].华北农学报,2019,34(1):54-60.doi:10.7668/hbnxb.201751053.
Que Z Q,Yu Z L,Shen C X.Expression patterns of annexin OsAnn8 and CRISPR/Cas9-mediated genome editing of rice under drought and low temperature condition[J]. Acta Agriculturae Boreali-Sinica,2019,34(1):54-60.
[4] Alsheikh M K,Heyen B J,Randall S K.Ion binding properties of the dehydrin ERD14 are dependent upon phosphorylation[J].Journal of Biological Chemistry,2003,278(42):40882-40889.doi:10.1074/jbc.M307151200.
[5] Taji T,Seki M,Yamaguchi-Shinozaki K,Kamada H,Giraudat J,Shinozaki K.Mapping of 25 drought-inducible genes, RD and ERD,in Arabidopsis thaliana[J].Plant Cell Physiol,1999,40(1):119-123.doi:10.1093/oxfordjournals.pcp.a029469.
[6] Liu Y H,Li H Y,Shi Y S,Song Y C,Wang T Y,Li Y.A maize early responsive to dehydration gene, ZmERD4, provides enhanced drought and salt tolerance in Arabidopsis[J].Plant Molecular Biology Reporter,2009,27(4):542-548.doi:10.1007/s11105-009-0119-y.
[7] 曹言勇,赵心萍,赵秋勇,张艳,唐保军,刘方方,王利锋,李会勇.玉米 ZmERD4 基因的分离及转基因株系的获得[J].河南农业科学,2011,40(12):43-47.doi:10.3969/j.issn.1004-3268. 2011.12.011.
Cao Y Y,Zhao X P,Zhao Q Y,Zhang Y,Tang B J,Liu F F,Wang L F,Li H Y.Isolation of ZmERD4 gene and generation of transgenic maize plants[J].Journal of Henan Agricultural Sciences,2011,40(12):43-47.
[8] Kiyosue T,Yamaguchy S K,Shinozaki K. Characterization of a cDNA for a dehydration-inducible gene that encodes a CLP A,B-like protein in Arabidopsis thaliaiana L[J]. Biochem Biophys Res Commun,1993,196(3):1214-1220.doi:10.1006/bbrc.1993.2381.
[9] 李横江,邵幼岩.玉米 ZmCIPK 基因的克隆及表达模式分析[J].河南农业科学,2014,43(1):15-18.doi:10.15933/j.cnki.1004-3268.2014.01.013.
Li H J,Shao Y Y.Cloning and expression patter analysis on ZmCIPK genes of maize[J].Journal of Henan Agricultural Sciences,2014,43(1):15-18.
[10] 朱丽萍,于壮,邹翠霞,李秋莉.植物逆境相关启动子及功能[J].遗传,2010,32(3):229-234.doi:10.3724/SP.J.1005.2010.00229.
Zhu L P,Yu Z,Zou C X,Li Q L. Plant stress-inducible promoters and their function[J]. Hereditas,2010,32(3):229-234.
[11] 孟繁君,黄骥,鲍永美,江燕,张红生.水稻TF ⅢA型锌指蛋白基因 ZFP207 的克隆和表达分析[J].遗传,2010,32(4):387-392.doi:10.3724/SP.J.1005.2010.00387.
Meng F J,Huang J,Bao Y M,Jiang Y,Zhang H S.Cloning and expression analysis of ZFP207 encoding a TF ⅢA-type zinc finger protein in rice[J]. Hereditas,2010,32(4):387-392.
[12] Froehlich J E,Wilkerson C G,Ray W K,Mcandrew R S,Osteryoung K W,Gage D A,Phinney B S. Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis[J].Jonrnal of Proteome Research,2003,2(4):413-425.doi:10.1021/pr034025j.
[13] Kiyosue T,Yamaguchi S K,Shinozaki K.Characterization of two cDNAs(ERD10) and (ERD14) corresponding to genes that respond rapidly to dehydration stress in Arabidopsis thaliana[J]. Plant and Cell Physiology,1994,35(2):225-231.doi:10.1093/oxfordjournals.pcp.a078588.
[14] Kariola T,Brader G,Helenius E,Li J,Heino P,Palva E T.EARLY RESPONSIVE TO DEHYDRATION 15,a negative regulator of abscisic acid responses in Arabidopsis[J].Plant Physiol,2006,142(4):1559-1573.doi:10.1104/pp.106.086223.
[15] Yuan F,Yang H M,Xue Y,Kong D D,Ye R,Li C J,Zhang J Y,Theprungsirikul L, Shrift T, Krichilsky B,Johnson D M, Swift G B, He Y K,Siedow J N,Pei Z M.OSCA1 mediates osmotic-stress-evoked Ca²⁺ increases vital for osmosensing in Arabidopsis[J].Nature,2014,514(7522):367-371.doi:10.1038/nature13593.
[16] Li Y S,Yuan Y,Wen Z H,Li Y H,Wang F,Zhu T,Zhuo W Q,Jin X,Wang Y D,Zhao H P,Pei Z M,Han S H.Genome-wide survey and expression analysis of the OSCA gene family in rice[J].BMC Plant Biology,2015,15(1):261.doi:10.1186/s12870-015-0653-8.
[17] 王傲雪,张可为,张瑶,陈秀玲.番茄OSCA基因家族鉴定及不同胁迫条件下表达分析[J].东北农业大学学报,2019,50(1):19-28.doi:10.19720/j.cnki.issn.1005-9369.2019.01.003.
Wang A X,Zhang K W,Zhang Y,Chen X L.Identification of tomato OSCA gene family and expression analysis under different stress conditions[J]. Journal of Northeast Agricultural University,2019,50(1):19-28.
[18] 吕广德.小麦 TaOSCA1.4 基因的克隆、标记开发和功能分析[D].泰安:山东农业大学,2015.
Lü G D.Cloning,molecular markers and function of the gene TaOSCA1.4 in common wheat[D].Taian:Shandong Agricultural University,2015.
[19] 陆月赏.玉米泛素延伸蛋白ZmERD16的基因克隆与功能分析[D]. 哈尔滨:东北农业大学,2010.doi:10.7666/d.y1787367.
Lu Y S.Identification and expression analysis of ZmERD16,a ubiquitin extension protein gene in maize(Zea mays L.)[D].Harbin:Northeast Agricultural University,2010.
[20] 袁珍,张鹏钰,王国瑞,曹丽茹,库丽霞,卫丽.玉米 ZmPP2C6-01 基因的克隆及表达分析[J].华北农学报,2019,34(2):8-18.doi:10.7668/hbnxb.201751477.
Yuan Z,Zhang P Y,Wang G R,Cao L R,Ku L X,Wei L.Cloning and expression of ZmPP2C6-01 gene in maize[J].Acta Agriculturae Boreali-Sinica,2019,34(2):8-18.
[21] 吕亚楠,余国武,黄玉碧.玉米AGPL2蛋白多克隆抗体制备及时空表达分析[J].华北农学报,2018,33(6):17-23.doi:10.7668/hbnxb.2018.06.003.
Lü Y N,Yu G W,Huang Y B.Preparation of polyclonal antibody of maize AGPL2 protein and analysis of its spatiotemporal expression analysis[J].Acta Agriculturae Boreali-Sinica,2018,33(6):17-23.

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玉米ERD基因的表达模式及ZmERD6基因的抗旱性分析

Expression Pattern of ERD Genes in Maize and Drought Resistance Analysis of ZmERD6 Gene

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玉米ERD基因的表达模式及ZmERD6基因的抗旱性分析

Expression Pattern of ERD Genes in Maize and Drought Resistance Analysis of ZmERD6 Gene

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