马铃薯<i>StNF-Y</i>核转录因子基因的克隆和生物信息学分析

doi:10.7668/hbnxb.201751585

摘要/Abstract

摘要： 为了研究马铃薯StNF-Y基因在非生物逆境中的作用与功能，在马铃薯抗青枯病基因型ED13中克隆了1个新的NF-YC转录因子（StNF-Y），并对其进行生物信息学分析，预测其生物学功能。结果表明，StNF-Y基因的全长cDNA为1 132 bp，其开放阅读框为693 bp，编码230个氨基酸，氨基酸分子式为C₁₁₁₂H₁₇₃₅N₃₁₇O₃₃₉S₁₀，等电点为5.37，含有13个磷酸化位点；StNF-Y蛋白分布在胞质中，无信号肽，属于亲水性蛋白，含有高度保守的结构域，属于BUR6 superfamily家族；二级结构显示，StNF-Y蛋白主要由45.65%的α-螺旋、5.65%的延伸链、45.22%的无规则卷曲和3.48%的β-转角组成。系统进化分析结果显示，马铃薯StNF-Y序列与番茄、辣椒和烟草NF-YC序列亲缘关系较近。此外，StNF-Y在多种植物胁迫中起作用。因此，推测StNF-Y可能参与马铃薯相关胁迫抗性的调节。研究结果可为以后进一步深入研究马铃薯StNF-Y的功能以及对逆境胁迫的抵抗机制提供理论依据。

关键词: 马铃薯, StNF-Y, 生物信息学, 电子克隆

Abstract: To study the role and function of potato StNF-Y gene under abiotic stress, a new NF-YC transcription factor(StNF-Y) was cloned from potato resistant to bacterial wilt genotype ED13. Bioinformatics analysis and biological function prediction showed that the full-length cDNA of StNF-Y was 1 132 bp, with an open reading frame of 693 bp, encoding 230 amino acids, with a formula of C₁₁₁₂H₁₇₃₅N₃₁₇O₃₃₉S₁₀ and isoelectric point of 5.37. It contained 13 phosphorylation sites, and StNF-Y protein was distributed in the cytoplasm, without signal peptide.It was a hydrophilic protein. StNF-Y protein contained a highly conserved domain, existed in the BUR6 superfamily. Secondary structure showed that the protein consisted mainly of 45.65% α-helix, 5.65% extended chain, 45.22% random coil and 3.48% β-turn. Phylogenetic analysis showed that the StNF-Y sequence of potato was closely related to the NF-YC sequences of tomato, pepper and tobacco. In addition, StNF-Y played a certain role in a variety of plant stresses, so it was speculated that StNF-Y might be involved in the regulation of potato-related stress resistance. The results would provide a theoretical basis for further research on the function of potato StNF-Y and its resistance mechanism to stress.

Key words: Potato, StNF-Y, Bioinformatics, In silico cloning

中图分类号:

Q78
S532

史丽, 吴亚茹, 王晓娟, 常燕楠, 庞鹏湘, 郜刚. 马铃薯StNF-Y核转录因子基因的克隆和生物信息学分析[J]. 华北农学报, 2019, 34(3): 31-37. doi: 10.7668/hbnxb.201751585.

SHI Li, WU Yaru, WANG Xiaojuan, CHANG Yannan, PANG Pengxiang, GAO Gang. Cloning and Bioinformatics Analysis of StNF-Y Nuclear Transcription Factor Gene in Potato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(3): 31-37. doi: 10.7668/hbnxb.201751585.

http://www.hbnxb.net/CN/Y2019/V34/I3/31

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马铃薯StNF-Y核转录因子基因的克隆和生物信息学分析

Cloning and Bioinformatics Analysis of StNF-Y Nuclear Transcription Factor Gene in Potato

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马铃薯StNF-Y核转录因子基因的克隆和生物信息学分析

Cloning and Bioinformatics Analysis of StNF-Y Nuclear Transcription Factor Gene in Potato

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