小花棘豆中毒对大鼠下丘脑-垂体-睾丸轴α-甘露糖苷酶的影响

doi:10.7668/hbnxb.2015.S1.066

华北农学报 ›› 2015, Vol. 30 ›› Issue (S1): 364-371. doi: 10.7668/hbnxb.2015.S1.066

小花棘豆中毒对大鼠下丘脑-垂体-睾丸轴α-甘露糖苷酶的影响

王帅, 贾琦珍, 张玲, 瞿胜, 陈根元

塔里木大学动物科学学院, 新疆生产建设兵团塔里木畜牧科技重点实验室, 新疆阿拉尔 843300

收稿日期:2015-09-24 出版日期:2015-12-28
通讯作者: 陈根元(1984-),男,湖南岳阳人,助理研究员,硕士,主要从事动物基因工程方面的研究.
作者简介:王帅(1984-),男,山西长治人,实验师,硕士,主要从事动物毒理学方面的研究.
基金资助:
国家自然科学基金项目(31460678);兵团塔里木畜牧科技重点实验室开放课题(HS201409)

Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Hypothalamus-pituitary-testicular Axis in Rats

WANG Shuai, JIA Qi-zhen, ZHANG Ling, QU Sheng, CHEN Gen-yuan

College of Animal Science, Tarim University, Key laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production & Construction Corps, Alar 843300, China

Received:2015-09-24 Published:2015-12-28

摘要/Abstract

摘要： 为探讨小花棘豆中毒对雄性大鼠下丘脑-垂体-睾丸轴α-甘露糖苷酶(AMA)的影响,进一步揭示小花棘豆的毒性作用机理.将144只雄性大鼠随机分为4组,即对照组和试验Ⅰ、Ⅱ、Ⅲ组.将小花棘豆全草粉碎后,按Ⅰ组添加15%(含苦马豆素30 mg/kg)、Ⅱ组添加30%(含苦马豆素60 mg/kg)、Ⅲ组添加45%(含苦马豆素90 mg/kg)的比例制作混合饲料,饲喂至典型中毒症状出现为止.攻毒后每7 d每组随机采集4只大鼠的下丘脑、垂体和睾丸,检测其AMA活性及表达的变化.结果表明,试验组及对照组大鼠下丘脑-垂体-睾丸轴高尔基体α-甘露糖苷酶Ⅱ(AMA1)和溶酶体α-甘露糖苷酶(AMA2)均有表达,但各试验组表达转录水平与对照组差异显著,试验Ⅰ组大鼠下丘脑-垂体-睾丸轴的 AMA1及AMA2 的表达均与对照组差异不显著(P>0.05),但试验Ⅱ组大鼠下丘脑-垂体-睾丸轴的 AMA1 以及试验Ⅲ组大鼠下丘脑-垂体-睾丸轴 AMA1、AMA2 的表达均显著低于对照(P<0.05),而且随着试验的进行抑制效果愈加明显.结果表明,小花棘豆中毒可影响大鼠下丘脑-垂体-睾丸轴AMA的活性及其基因的转录表达.

关键词: 小花棘豆, 苦马豆素, &alpha, -甘露糖苷酶, 下丘脑-垂体-睾丸轴

Abstract: The aim of this study was to investigate the effects of content and transcriptional expression of α-mannosidase(AMA) in hypothalamus-pituitary-testicular axis poisoning by Oxytropis glabra DC,and reveal the toxicity mechanism of O.glabra DC in future.144 male rats were randomly and equally divided into 4 groups(the control group and experimental groupⅠ,Ⅱ,Ⅲ),the dried plant of O.glabra DC was comminnuted,and different amounts of the grass powder(15%,30%and 45% the corresponding swainsonine content was 30,60 and 90 mg/kg) were mixed with the feeds in the three experimental groups.The rats consumed the forages freely until typical symptoms were observed.4 rats were selected randomly from each group every 7 day respectively,the hypothalamus,pituitary and testis were collected and the contents of AMA and its mRNA expression in different encephalic regions were measured.It was found that AMA in golgi and lysosomal expressed in hypothalamus-pituitary-gonad of all experimental groups, O.glabra DC inhibited the expression of hypothalamus-pituitary-testicular axis AMA1 and AMA2 mRNA,and the difference was obvious.The difference was not obvious in experimental groupⅠ and control group in hypothalamus-pituitary-testicular axis(P>0.05),the expression of AMA1 and AMA2 mRNA decreased in experimental group Ⅲ and AMA1 mRNA decreased in experimental groupⅡ was significant lower than control group in hypothalamus-pituitary-testicular axis(P<0.05),and it was significant lower than control group.The results showed that O.glabra DC has remarkable effects on content and transcriptional expression of AMA in male rat hypothalamus-pituitary-testicular axis,in a time-effect as a dose-effect relationship.

Key words: Oxytropis glabra DC, Swainsonine, α-mannosidase, Hypothalamus-pituitary-testicular axis

中图分类号:

S856.9

王帅, 贾琦珍, 张玲, 瞿胜, 陈根元. 小花棘豆中毒对大鼠下丘脑-垂体-睾丸轴α-甘露糖苷酶的影响[J]. 华北农学报, 2015, 30(S1): 364-371. doi: 10.7668/hbnxb.2015.S1.066.

WANG Shuai, JIA Qi-zhen, ZHANG Ling, QU Sheng, CHEN Gen-yuan. Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Hypothalamus-pituitary-testicular Axis in Rats[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 364-371. doi: 10.7668/hbnxb.2015.S1.066.

http://www.hbnxb.net/CN/Y2015/V30/IS1/364

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Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Hypothalamus-pituitary-testicular Axis in Rats

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小花棘豆中毒对大鼠下丘脑-垂体-睾丸轴α-甘露糖苷酶的影响

Effects of Oxytropis glabra DC Poisoning on α-mannosidase of Hypothalamus-pituitary-testicular Axis in Rats

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