植物抗炭疽病相关基因的研究进展

doi:10.7668/hbnxb.2015.S1.063

摘要/Abstract

摘要： 植物在受到炭疽病菌侵袭的时候,会引起植物体内的一系列抗炭疽病基因表达,从而提高了植物对炭疽病的抵抗能力.随着科学技术的飞速发展及生物技术水平的不断提高,植物抗炭疽病基因成为研究的热点.本研究主要从植物抗炭疽病基因的遗传特点、基因定位、克隆、转化及抗病特异标记等方面的研究进展进行总结,并在植物抗炭疽病基因挖掘及转基因等方面提出了一些个人见解.

关键词: 植物, 抗炭疽病, 基因, 研究进展

Abstract: The expression of a series of resistant genes may be induced when the Colletotrichum infect the plants,thus the resistance to plant anthracnose could be enhanced.With the rapid development of science technology and biotechnology,the research of anthracnose resistant genes in plant has become a hotspot.This paper summarized the research progress in the genetic characteristics,genes mapping,cloning,genetic transformation and specific resistant markers of the anthracnose resistant genes in plant,and also put forward some personal opinions on gene exploring and transformation of anthracnose resistant genes.

Key words: Plant, Resistance anthracnose, Genes, Research progress

中图分类号:

S324.4+4

谢冬微, 路颖, 杨学, 赵德宝, 孙健. 植物抗炭疽病相关基因的研究进展[J]. 华北农学报, 2015, 30(S1): 348-354. doi: 10.7668/hbnxb.2015.S1.063.

XIE Dong-wei, LU Ying, YANG Xue, ZHAO De-bao, SUN Jian. Research Progress on Related Genes of Resistance to Anthracnose in Plant[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 348-354. doi: 10.7668/hbnxb.2015.S1.063.

http://www.hbnxb.net/CN/Y2015/V30/IS1/348

参考文献

[1] Tode H J.Fungi mecklenbergensis selecti[M].Luneburg:Ascophora Tode,1790:1-46.
[2] Von Jacob Sturm.Deutschlands flora in Abbildungen nachder Naturmit Beschreibungen[M].Nrnberg:Gedrukt auf kosfen des verfassers,1831,12:33-64.
[3] von Arx J A.Die artender gattung Colletotrichum.Cda,[J].Phytopathology,1957,29:413-468.
[4] Sutton B C.The coelomycetes[M].England:CMI Kew Surrey,1980:127-130.
[5] 王晓鸣.炭疽菌属(Colletotrichcum Cda)的现代分类学和陕西省炭疽菌属的种[D].西安:西北农业大学,1985.
[6] 杨友联,刘永翔,刘作易.炭疽菌属真菌分类学研究进展[J].贵州农业科学,2011,39(1):152-157.
[7] 王惠哲,李淑菊,刘秀峰,等.与黄瓜抗炭疽病相关基因连锁的AFLP标记的筛选[J].园艺学报,2007,34(1):213-216.
[8] Sun C Y,Mao S L,Zhang Z H,et al.Resistances to anthracnose (Colletotrichum acutatum) of Capsicum mature green and ripe fruit are controlled by a major dominant cluster of QTLs on chromosome P5[J].Scientia Horticulturae,2015,181:81-88.
[9] 陈明丽.普通菜豆SSR标记开发及抗炭疽病基因分子标记定位[D].北京:中国农业科学院,2011.
[10] 牛晓伟,唐宁安,范敏,等.西瓜抗炭疽病的遗传分析和抗性基因定位研究[J].核农学报,2014,28(8):1365-1369.
[11] 万怡震.中国葡萄属野生种抗病性及抗病基因RAPD标记作图研究[D].杨凌:西北农林科技大学,2003.
[12] 王坤,王晓鸣,朱振东,等.以SSR标记对普通菜豆抗炭疽病基因定位[J].作物学报,2009,35(3):432-437.
[13] 陈明丽,王兰芬,王晓鸣,等.利用分子标记定位普通菜豆抗炭疽病基因[J].作物学报,2011,37(12):2130-2135.
[14] 李静.草莓抗炭疽病遗传图谱构建和抗性相关基因的克隆与表达分析[D].南京:南京农业大学,2012.
[15] Upadhyaya H D,Wang Y H,Sharma R,et al.Identification of genetic markers linked to anthracnose resistance in sorghum using association analysis[J].Theoretical and Applied Genetics,2013,126(6):1649-1657.
[16] Cuevas H E,Prom L K,Erpelding J E.Inheritance and molecular mapping of anthracnose resistance genes present in sorghum line SC112-14[J].Molecular Breeding,2014,34(4):1943-1953.
[17] Mackie J M,Musial J M,Armour D J,et al.Identification of QTL for reaction to three races of Colletotrichum trifolii and further analysis of inheritance of resistance in autotetraploid lucerne[J].Theoretical and Applied Genetics,2007,114(8):1417-1426.
[18] Melotto M,Vallejo V,Awale H,et al.D.Use of RAPD and AFI.P analysis to tag the Co-1 gene conditionln resistance to bean anthracnose[J].Annu Rpt Bean Improv Coop,2000,43:84-85.
[19] Mastenbroek C.A breeding programme for resistance to anthracnose in dry shell haricot beans,based on a new gene[J].Euphytica,1960,9(2):177-184.
[20] Fouilloux G.Bean anthracnose:New genes of resistance[J].Annu Rpt Bean Improv Coop,1976,19:36-37.
[21] Alzate-Marin A L,Souza K A,Silva M G M,et al.Genetic characterization of anthracnose resistance genes Co-43 and Co-9 in common bean cultivar tlalneplantla 64 (PI207262)[J].Euphytica,2007,154:1-8.
[22] Young R A,Kelly J D.Characterization of the genetic resistance to Colletotrichum lindemuthianum in common bean differential cultivars[J].Plant Dis,1996,80:650-654.
[23] Alzate-Matin A L,Costa M R,Arruda K M,et al.Characterization of the anthracnose resistance locus present in cultivar Ouro Negro(Honduras 35)[J].Euphytica,2003,133:165-169.
[24] Inoue Y,Mori R,Takahashi Y,et al.Identification and molecular mapping of a wheat gene for resistance to an unadapted isolate of Colletotrichum cereale[J].Phytopathology,2013,103(6):575-582.
[25] Biruma M,Martin T,Fridborg I,et al.Two loci in sorghum with NB-LRR encoding genes confer resistance to Colletotrichum sublineolum[J].Theoretical and Applied Genetics,2012,124(6):1005-1015.
[26] Birker D,Heidrich K,Takahara H,et al.A locus conferring resistance to Colletotrichum higginsianum is shared by four geographically distinct Arabidopsis accessions[J].The Plant Journal:for Cell and Molecular Biology,2009,60(4):602-613.
[27] Narusaka Y,Narusaka M,Park P,et al.RCH1,a locus in Arabidopsis that confers resistance to the hemibiotrophic fungal pathogen Colletotrichum higginsianum[J].Molecular Plant-Microbe Interactions,2004,17(7):749-762.
[28] Lee J,Hong J H,Do J W,et al.Identification of QTLs for resistance to anthracnose to two Colletotrichum species in pepper[J].Journal of Crop Science and Biotechnology,2010,13(4):227-233.
[29] Yang S,Gao M,Deshpande S,et al.Genetic and physical localization of an anthracnose resistance gene in Medicago truncatula[J].Theoretical and Applied Genetics,2007,116(1):45-52.
[30] 申建斌.Mineirao柱花草抗炭疽病候选基因MSRA的克隆及其序列分析[D].海口:华南热带农业大学,2007.
[31] Li L,Zhu F Y,Liu H J,et al.Isolation and expression analysis of defense-related genes in sorghum-Colletotrichum sublineolum interaction[J].Physiological and Molecular Plant Pathology,2013,84:123-130.
[32] Cai Z,Li G,Lin C,et al.Identifying pathogenicity genes in the rubber tree anthracnose fungus Colletotrichum gloeosporioides through random insertional mutagenesis[J].Microbiological Research,2013,168(6):340-350.
[33] Yang S,Gao M,Xu C,et al.Alfalfa benefits from Medicago truncatula:the RCT1 gene from M.truncatula confers broad-spectrum resistance to anthracnose in alfalfa[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(34):12164-12169.
[34] Broglie K E,Butler K H,Butruille M G,et al.Polynucleotides and methods for making plants resistant to fungal pathogens[P].US.Patent,20,060,223,102.2009.
[35] 张庆雨,刘芳春,段可,等.水杨酸对草莓炭疽病响应基因FaNBS20表达的影响[J].园艺学报,2014,41(1):53-62.
[36] 陈弟,谭志琼,殷晓敏,等.香蕉果实炭疽病病程相关基因cDNA片段克隆[J].植物病理学报,2009(3):325-327.
[37] 王秀菊.辣椒多聚半乳糖醛酸酶抑制蛋白基因克隆及功能研究[D].泰安:山东农业大学,2011.
[38] 石静.棉花 GhMPK16 基因的分离与功能分析[D].兰州:甘肃农业大学,2010.
[39] 孙静.棉花 GhWRKY11 基因的分离及其功能分析[D].泰安:山东农业大学,2013.
[40] 刘春.西柏利亚蓼 PsGSTU1、PsCSase1、PsnsLTP 基因的抗逆功能分析[D].哈尔滨:东北林业大学,2011.
[41] 谢玉明,马先锋,韩健,等.转 chit42 基因柠檬的分子鉴定及抗炭疽病研究[J].果树学报,2008,25(5):695-698.
[42] 柳忠玉,赵树进.转PcRS基因拟南芥的抗炭疽病研究[J].生物技术通报,2014(10):107-112.
[43] Liu Z,Zhuang C,Sheng S,et al.Overexpression of a resveratrol synthase gene(PcRS)from in transgenic causes the accumulation of-piceid with antifungal activity[J].Plant Cell Reports,2011,30(11):2027-2036.
[44] Silva K,Brunings A M,Peres N A,et al.Enhance resistance against Colletotrichum gloeosporioides in strawberry by overexpressing the Arabidopsis NPR 1 gene[J].Phytopathology,2013,103(6,2):134.
[45] Jia Z C,Gou J Q,Sun Y M,et al.Enhanced resistance to fungal pathogens in transgenic Populus tomentosa Carr.by overexpression of an nsLTP-like antimicrobial protein gene from motherwort (Leonurus japonicus)[J].Tree Physiology,2010,30(12):1599-1605.
[46] 李明.类黄酮调控基因 AtMYB12 的异源表达及其对植物抗病性的贡献[D].泰安:山东农业大学,2012.
[47] Seo H H,Park S,Park S,et al.Overexpression of a defensin enhances resistance to a fruit-specific anthracnose fungus in pepper[J].PLoS One,2014,9(5):e97936.
[48] An C F,Mou Z L.Salicylic acid and its function in plant immunity[J].Journal of Integrative Plant Biology,2011,53(6):412-428.
[49] Guidarelli M,Zoli L,Orlandini A,et al.The mannose-binding lectin gene FaMBL1 is involved in the resistance of unripe strawberry fruits to Colletotrichum acutatum[J].Molecular Plant Pathology,2014,15(8):832-840.
[50] Tang Y,Kuang J F,Wang F Y,et al.Molecular characterization of PR and WRKY genes during SA-and MeJA-induced resistance against Colletotrichum musae in banana fruit[J].Postharvest Biology and Technology,2013,79:62-68.
[51] Liu C L,Ruan Y,Lin Z J,et al.Antagonism between acibenzolar-S-methyl-induced systemic acquired resistance and jasmonic acid-induced systemic acquired susceptibility to Colletotrichum orbiculare infection in cucumber[J].Physiological and Molecular Plant Pathology,2008,72(4/6):141-145.
[52] Cao S F,Zheng Y H,Yang Z F,et al.Effect of methyl jasmonate on the inhibition of Colletotrichum acutatum infection in loquat fruit and the possible mechanisms[J].Postharvest Biology and Technology,2008,49(2):301-307.
[53] Shi J,An H L,Zhang L,et al. GhMPK7,a novel multiple stress-responsive cotton group C MAPK gene,has a role in broad spectrum disease resistance and plant development[J].Plant Molecular Biology,2010,74(1/2):1-17.
[54] Paran I,Michelmore R W.Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce[J].Theoretical and Applied Genetics,1993,85(8):985-993.
[55] 古瑜,韩启厚,王武台,等.菜豆抗炭疽病基因SCAR标记在品种抗性鉴定中的应用[J].园艺学报,2011,38(5):911-920.
[56] 赵晓彦,王晓鸣,朱振东,等.普通菜豆抗炭疽病基因鉴定与分子标记[J].植物遗传资源学报,2006,7(1):95-99,105.
[57] 李淑菊,王惠哲,霍振荣,等.黄瓜抗炭疽病相关基因AFLP标记的SCAR转换[J].园艺学报,2008,35(1):123-126.
[58] 王惠哲,管炜,李淑菊.与黄瓜抗炭疽病相关基因连锁的基因片段及SCAR标记[J].华北农学报,2011,26(1):200-203.
[59] 吕香玲,王邦太,史利玉,等.玉米抗甘蔗花叶病毒SCAR分子标记开发[J].中国农业科学,2009,42(6):1980-1987.
[60] 王坤,王晓鸣,朱振东,等.普通菜豆抗炭疽病地方品种的朊蛋白标记分析[J].植物遗传资源学报,2009,10(2):169-174.
[61] Adam-Blondon A F,Sévignac M,Bannerot H,et al.SCAR,RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean[J].Theoretical and Applied Genetics,1994,88(6/7):865-870.
[62] Geffroy V,Creusot F,Falquet J,et al.A family of LRR sequences in the vicinity of the Co-2 locus for anthracnose resistance in Phaseolus vulgaris and its potential use in marker-assisted selection[J].Theoretical and Applied Genetics,1998,96(3/4):494-502.
[63] Young R A,Kelly J D.RAPD markers flanking the Are gene for anthracnose resistance in common bean[J].Journal of the American Society for Horticultural Science,1996,121(1):37-41.
[64] Young R A,Melotto M,Nodari R O,et al.Marker-assisted dissection of the oligogenic anthracnose resistance in the common bean cultivar,G2333[J].Theoretical and Applied Genetics,1998,96(1):87-94.
[65] Awale H E,Ismail S M,Vallejo V A,et al.SQ4 SCAR marker linked to the Co-2 gene on B11 appears to be linked to the Ur-11 gene[J].Annu Rep Bean Improv Coop,2008,51:174-175.
[66] Kelly J D,Gepts P,Miklas P N,et al.Tagging and mapping of genes and QTL and molecular marker-assisted selection for traits of economic importance in bean and cowpea[J].Field Crops Research,2003,82(2/3):135-154.
[67] de Queiroz V T,de Sousa C S,Costa M R,et al.Development of SCAR markers linked to common bean anthracnose resistance genes Co-4 and Co-6[J].Annu Rep Bean Improv Coop,2004,47:249-250.
[68] Vallejo V,Kelly J D.Development of a SCAR marker linked to Co-5 in common bean[J].Annu Rep Bean Improv Coop,2001,44:121-122.
[69] Campa A,Rodr guez-Su rez C,Pa eda A,et al.Bean anthracnose resistance gene Co-5,is located in linkage group B7[J].Annu Rep Bean Improv Coop,2005,48:68-69.
[70] De Vigo B M,Rodriguez C,Pa eda A,et al.Development of a SCAR marker linked to Co-9 in common bean[J].Annu Rep Bean Improv Coop,2002,45:116-117.
[71] Alzate-Marin A L,Costa M R,Arruda K M,et al.Characterization of the anthracnose resistance gene present in Ouro Negro(Honduras 35)common bean cultivar[J].Euphytica,2003,133(2):165-169.
[72] Miklas P N,Delorme R,Stone V,et al.Bacterial,fungal,and viral disease resistance loci mapped in a recombinant inbred common bean population(dorado'/XAN 176)[J].Journal of the American Society for Horticultural Science,2000,125(4):476-481.
[73] Rodr guez-Su rez C,Ferreira J J,Campa A,et al.Molecular mapping and intra-cluster recombination between anthracnose race-specific resistance genes in the common bean differential cultivars Mexico 222 and Widusa[J].Theoretical and Applied Genetics,2008,116(6):807-814.
[74] Geetha S B,Subbareddy S,Satishchandra P,et al.Morphological and molecular screening of French bean(Phaseolus vulgaris L.)germplasm using SCAR markers for Colletotrichum lindemuthianum(Sacc.and Magn.)Scrib.causing anthracnose resistance[J].Arch Phytopathology Plant Protect,2013,46(1):84-97.

选择文件类型/文献管理软件名称

选择包含的内容