摘要： 为了筛选抗穗发芽品系,利用 Vp-1 基因的STS标记 Vp1B3 检测了来自河南省新乡市的新麦系列小麦新品系112个,并于收获期进行了室内整穗发芽试验,结果表明,112个小麦品系中 Vp-1Ba (感穗发芽)、 Vp-1Bb (抗穗发芽)和 Vp-1Bc (抗穗发芽)基因型的频率分别为60.71%,0.89%和38.39%,以 Vp-1Ba 基因型为主。 Vp-1Ba 基因型品系穗发芽率(24.42%)极显著高于 Vp-1Bc 基因型品系(16.55%)。同一亲本组合选育出的品系的穗发芽率有很大差异,但 Vp-1 基因类型基本一致。同一 Vp-1 类型品系间的穗发芽率有很大差异,故在使用 Vp1B3 标记时需要与整穗发芽法结合使用。

关键词: 小麦, 穗发芽, Vp-1 基因, Vp1B3 标记

Abstract: In order to get wheat cultivars with resistance to pre-harvest sprouting(PHS) and provide theory evidence for anti-PHS breeding in Xinxiang region,a functional marker Vp1B3 was used to investigate 112 wheat cultivars breed by Xinxiang Academy of Agricultural Sciences.Germination percentages on ear of those cultivars were counted indoor at the same time.The results's indicated that the frequencies of cultivars with the genotype Vp-1Ba (susceptible to PHS), Vp-1Bb (resistant to PHS) and Vp-1Bc (resistant to PHS) were 60.71%,0.89% and 38.39%,respectively.The genotype Vp-1Ba was the major genotype in those wheat cultivars.The germination percentages of cultivars with genotype Vp-1Ba (24.42%) and genotype Vp-1Bc (16.55%) was remarkable different.The germination percentages among cultivars from the same parental cross varied greatly,but genotypes of Vp-1 were almost identical among cultivars from the same parental cross.Pre-harvest sprouting rate of cultivars with the same Vp-1 genotype also varied greatly,therefore the application of Vp1B3 marker should be combined with the test of germination on ear.

Key words: Wheat, Pre-harvest sprouting, Vp-1 gene, Vp1B3 marker

中图分类号: 

• S512.01