华北农学报 ›› 2015, Vol. 30 ›› Issue (2): 150-155. doi: 10.7668/hbnxb.2015.02.027

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枣与酸枣的SSR遗传多样性研究

张鹏飞1, 尉东峰2, 刘亚令3, 宋美玲3, 张茹3, 雍鹏4   

  1. 1. 山西农业大学 园艺学院, 山西 太谷 030801;
    2. 山西省阳高县林业局, 山西 阳高 038100;
    3. 山西农业大学 生命科学学院, 山西 太谷 030801;
    4. 山西省林业科学研究院, 山西 太原 030012
  • 收稿日期:2014-12-17 出版日期:2015-04-28
  • 通讯作者: 雍鹏(1978-),男,山西交城人,工程师,硕士,主要从事枣树良种选育研究。
  • 作者简介:张鹏飞(1979-),男,山西阳城人,讲师,硕士,主要从事果树生长与发育调控研究。
  • 基金资助:
    山西省科技攻关项目(20110311012-1)

Genetic Diversity of Ziziphus jujuba Mill.and Z.jujuba var. spinosa Based on SSR Markers

ZHANG Peng-fei1, YU Dong-feng2, LIU Ya-ling3, SONG Mei-ling3, ZHANG Ru3, YONG Peng4   

  1. 1. College of Horticulture, Shanxi Agricultural University, Taigu 030801, China;
    2. Forestry Bureau in Yanggao County, Yanggao 038100, China;
    3. College of Life Science, Shanxi Agricultural University, Taigu 030801, China;
    4. Shanxi Academy of Forestry, Taiyuan 030012, China
  • Received:2014-12-17 Published:2015-04-28

摘要: 为了探讨枣与酸枣资源的遗传多样性以及两者的亲缘关系,采用7对SSR引物,对16份枣品种(系)和17份酸枣的遗传多样性进行分析。结果表明:16份枣样品共扩增出56个等位基因,有效等位基因数(Ne)为3.798~10.000,平均为6.953,Shannon's信息指数(I)为1.984,期望杂合度(He)为0.837;17份酸枣样品扩增后共检测出73个等位基因,等位基因的有效数目(Ne)为3.273~11.840,平均为7.398,Shannon's信息指数(I)为2.105,期望杂合度(He)为0.843;枣和酸枣的遗传多样性都很丰富,酸枣的遗传多样性水平高于枣;GenAlEx分析得出,枣和酸枣居群种间遗传分化系数(Fst)为0.055,居群种间基因流(Nm)平均值为4.295,说明居群间基因交流比较频繁。NTSYSpc聚类分析表明,SSR分子标记可以将枣和酸枣划分为枣类、酸枣类和过渡类3个类群。

关键词: 枣, 酸枣, SSR, 遗传多样性

Abstract: To know the genetic diversity and the genetic relationship of Ziziphus jujuba Mill. and Z.jujuba var.spinosa germplasm resources, 7 pairs of SSR molecular markers were used to analyze the genetic diversity of 16 Ziziphus jujuba Mill.and 17 Z.jujuba var. spinosa in this experiment.The results showed that 56 alleles were amplified in 16 jujbe samples, and number of effective alleles (Ne)ranged from 3.798 to 10.000 and averaged at 6.953, Shannon's information index (I) was 1.984, expected heterozygosity (He) was 0.837.Moreover, 73 alleles were amplified in 17 wild jujube samples, Ne ranged from 3.273 to 11.840 and averaged at 7.398, I was 2.105, He was 0.843.So, genetic diversity of both of jujube and wild jujube were rich, and the wild jujube level of genetic diversity was higher than that of jujube.And, the GenALEx analysis showed that gene flow between populations exchanged frequently, and population genetic differentiation percentage (Fst) of jujube and wild jujube was 0.055, the average value of gene flow among populations (Nm) was 4.295.Furthermore, NTSYSpc cluster analysis showed that jujube and wild jujube could be divided into three groups of jujube, wild jujube and transition group using SSR molecular markers.

Key words: Ziziphus jujuba Mill., Z.jujuba var.spinosa, SSR, Genetic relationship

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引用本文

张鹏飞, 尉东峰, 刘亚令, 宋美玲, 张茹, 雍鹏. 枣与酸枣的SSR遗传多样性研究[J]. 华北农学报, 2015, 30(2): 150-155. doi: 10.7668/hbnxb.2015.02.027.

ZHANG Peng-fei, YU Dong-feng, LIU Ya-ling, SONG Mei-ling, ZHANG Ru, YONG Peng. Genetic Diversity of Ziziphus jujuba Mill.and Z.jujuba var. spinosa Based on SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 150-155. doi: 10.7668/hbnxb.2015.02.027.

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