摘要： 为进一步挖掘白刺属植物果实中花青素成分，以采自内蒙古两个地点的西伯利亚白刺果实为材料，利用高效液相色谱（HPLC-DAD）技术，建立了定性定量分析西伯利亚白刺果实中花青素的方法，同时结合离子阱多级质谱（MSⁿ）技术，从西伯利亚白刺果实甲醇提取物中鉴定出12种花青素组分，分别为：矢车菊素3-槐糖苷、矢车菊素3-戊糖苷、矮牵牛素3-芸香糖-葡萄糖苷、芍药素3-槐糖苷、矮牵牛素3-鼠李糖苷、锦葵素3-阿拉伯糖苷、芍药素3-己糖苷、矢车菊素3-顺式-香豆酰二葡萄糖苷、矢车菊素3-反式-香豆酰二葡萄糖苷、天竺葵素3-香豆酰二葡萄糖苷、芍药素3-丙二酰葡萄糖苷、矮牵牛素3-香豆酰葡萄糖苷。利用所建立的方法，分析并比较了3个不同成熟阶段和2个采集点样品中花青素组成和含量的差异。

关键词: 西伯利亚白刺, 果实, 成熟阶段, 花青素, 高效液相色谱, 离子阱质谱

Abstract: In the present study,an HPLC method was established for qualitative and quantitative analysis of the fruits of Nitraria sibirica based on the HPLC-DAD/TRAP-MS technique.The chemical structure of 12 anthocyanin components were identified,including:Cyanidin 3-O-sophoroside,Cyanidin 3-O-hexose,Cyanidin 3-O-(cis-p-coumaroyl)-diglucoside,Cyanidin 3-O-(trans-p-coumaroyl)-diglucoside,Petunidin 3-O-rutinoside-glucose,Petunidin 3-O-rhamnoside,Petunidin 3-O-(p-coumaroyl)-glucoside,Peonidin 3-O-hexose,Peonidin 3-O-sophoroside,Peonidin 3-O-malonyl-glucoside,Pelargonidin 3-O-(p-coumaroyl)-diglucoside and Malvidin 3-O-arabinose.In addition,the content of the anthocyanin components were also determined.It provides the scientific basis for the further exploitation and utilization of anthocyanins in Nitraria sibirica.

Key words: Nitraria sibirica, Fruit, Ripening stage, Anthocyanins, HPLC-DAD, Trap-MS

中图分类号: 

• Q946.83+6