蓝莓<em>VcMYB</em>启动子克隆及其功能初步分析

doi:10.7668/hbnxb.2017.02.003

摘要/Abstract

摘要： 为探究VcMYB启动子在转录过程中如何发挥调控作用，利用FPNI-PCR法从蓝莓中克隆到调控原花青素合成相关的转录因子VcMYB的768 bp启动子序列。用PLACE和Plant CARE在线启动子预测工具分析了该启动子，结果表明其序列中存在启动子的基本元件CAAT-box和TATA-box，还包含一系列的响应元件，如光响应元件、低温响应元件、防御与胁迫响应元件和茉莉酸甲酯响应元件等。为进一步分析该启动子的功能，构建了该基因启动子与GUS基因融合的植物表达载体VcMYBpro：：GUS，并用农杆菌转化拟南芥。对转基因拟南芥进行GUS组织化学染色分析，结果表明该VcMYB启动子能驱动GUS基因在转基因拟南芥中表达，并且经脱落酸（ABA）、4℃低温、LED光照和持续光照处理后，转基因拟南芥中GUS的表达活性增强，推测该基因受ABA、低温和光的调控。

关键词: 蓝莓, VcMYB, 启动子, GUS基因, 原花青素

Abstract: In order to study the regulatory role of VcMYB promoter during the transcription,the promoter sequence of the VcMYB gene,regulated the proanthocyanidin synthesis genes from highbush blueberry,was cloned by the methods of FPNI-PCR.The 768 bp promoter sequence was analyzed by PLACE and Plant CARE.The analysis revealed that the promoter sequence contained CAAT-box,TATA-box and some cis-acting element such as light responsive element,cis-acting element involved in low-temperature,MeJA,defense and stress responsiveness.In order to study the function of VcMYB promoter,a promoter-reporter vector VcMYBpro::GUS was constructed,and introduced into Arabidopisis thaliana by Agrobacterium-mediated method.The result of GUS histochemical staining showed that GUS reporter gene expression was driven by VcMYB promoter in transgenic plants,and GUS enzyme activity of transgenic plants has increased after different treatments,including ABA (Abscisic acid),4℃ low temperature,LED light and continuous light.These results indicated that VcMYB promoter was regulated by ABA,low temperature and light.

Key words: Vaccinium corymbosum, VcMYB, Promoter, GUS gene, Proanthocyanidin

中图分类号:

Q78
S668.03

史文君, 滕珂, 陈露, 侯智霞, 苏淑钗. 蓝莓VcMYB启动子克隆及其功能初步分析[J]. 华北农学报, 2017, 32(2): 14-20. doi: 10.7668/hbnxb.2017.02.003.

SHI Wenjun, TENG Ke, CHEN Lu, HOU Zhixia, SU Shuchai. Cloning and Functional Analysis of VcMYB Promoter from Vaccinium corymbosum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(2): 14-20. doi: 10.7668/hbnxb.2017.02.003.

http://www.hbnxb.net/CN/Y2017/V32/I2/14

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蓝莓VcMYB启动子克隆及其功能初步分析

Cloning and Functional Analysis of VcMYB Promoter from Vaccinium corymbosum

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蓝莓VcMYB启动子克隆及其功能初步分析

Cloning and Functional Analysis of VcMYB Promoter from Vaccinium corymbosum

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