四川白鹅,i>OAZ1和<em>OAZ2</em>基因组织差异表达的研究

doi:10.7668/hbnxb.2014.01.010

华北农学报 ›› 2014, Vol. 29 ›› Issue (1): 50-54. doi: 10.7668/hbnxb.2014.01.010

所属专题： 生物技术；

四川白鹅,i>OAZ1和OAZ2基因组织差异表达的研究

易治鑫, 姜冬梅, 康波

四川农业大学动物科技学院, 四川雅安 625014

收稿日期:2013-11-16 出版日期:2014-02-28
通讯作者: 姜冬梅(1978-)，女，黑龙江海林人，实验师，硕士，主要从事动物生产研究;康波(1978-)，男，黑龙江林口人，副教授，博士，主要从事动物生理与环境生理、家禽遗传育种与繁殖领域研究.
作者简介:易治鑫(1991-)，男，重庆江津人，主要从事特种经济动物养殖研究.
基金资助:
国家自然科学基金项目(31201798)；高等学校博士学科点专项科研基金资助课题项目(20105103120003)

Study on Differential Expression of OAZ1 and OAZ2 Gene in Various Tissues of the Sichuan White Goose

YI Zhi-xin, JIANG Dong-mei, KANG Bo

College of Animal Science and Technology,Sichuan Agricultural University,Yaan 625014,China

Received:2013-11-16 Published:2014-02-28

摘要/Abstract

摘要： 为阐明四川白鹅不同组织中OAZ1和OAZ2基因表达规律,应用实时荧光定量PCR技术检测四川白鹅13种组织中OAZ1和OAZ2基因相对表达量,分析不同组织中OAZ1表达量与OAZ2表达量比值(OAZ1/OAZ2)的变化规律。结果表明,四川白鹅大脑和脾脏中OAZ1高度表达,分别是心脏的8.74,8.45倍(P <0.05);胸肌、腿肌和卵巢组织中表达量最低,仅为心脏的0.15,0.42,0.46倍。视网膜中OAZ2高度表达,是心脏的6.79倍(P <0.05);肝脏、胸肌和腿肌组织中表达量最低,分别是心脏的0.47,0.08,0.19倍(P <0.05)。四川白鹅胸肌和腿肌中OAZ2表达占优势,其余各组织中OAZ1表达占优势。卵巢组织中OAZ1/OAZ2的值最高,其次是小脑组织,分别为26.62和17.72;胸肌和腿肌中OAZ1/OAZ2最低,分别为0.33和0.75。四川白鹅OAZ1和OAZ2基因表达具有组织特异性,不同组织中OAZ1/OAZ2值不同,提示不同组织中OAZ1和OAZ2的调控功能可能存在差异。

关键词: 四川白鹅, OAZ1, OAZ2, 基因表达, OAZ1/OAZ2

Abstract: The objective of this work was to elucidate the expression profiling of OAZ1 and OAZ2 in various tissues of Sichuan white geese. The relative abundance of OAZ1 and OAZ2 mRNA in thirteen tissues of Sichuan white geese were determined by quantitative real-time PCR. The ratio between the amounts of OAZ1 and OAZ2 gene expression was assessed. OAZ1 mRNA was most highly expression in brain and spleen,8. 74-fold and 8. 45-fold,respectively,compared with that in heart(P <0. 05). The amount of OAZ1 gene expression in breast,thigh muscle and ovarian tissues was lower,0. 15-fold,0. 42-fold and 0. 46-fold,respectively,compared with that in heart. The level of OAZ2 mRNA expression in retina was higher 6. 79-fold compared with that in heart(P <0. 05). The relative abundance of OAZ2 in liver,breast and thigh muscle was lower,0. 47-fold,0. 08-fold and 0. 19-fold,respectively,compared with that in heart(P <0. 05). OAZ2 mRNA was more abundant than that of OAZ1 in breast and thigh muscle. OAZ1 mRNA was more abundant than that of OAZ2 in other determined tissues in the experiment. The highest OAZ1 / OAZ2 ratio was found in ovary and cerebellum(26. 62 and 17. 72,respectively),whereas the lowest one was in breast and thigh muscle(0. 33 and 0. 75,respectively). OAZ1 and OAZ2 mRNA are ubiquitously and tissue-specificity expressed. The OAZ1 / OAZ2 ratio is dependent on the different tissue of Sichuan white goose. OAZ1 and OAZ2 may exert differently regulatory function in various tissues.

Key words: Sichuan white goose, OAZI, OAZ2, Gene expression, OAZ1/OAZ2

中图分类号:

易治鑫, 姜冬梅, 康波. 四川白鹅,i>OAZ1和OAZ2基因组织差异表达的研究[J]. 华北农学报, 2014, 29(1): 50-54. doi: 10.7668/hbnxb.2014.01.010.

YI Zhi-xin, JIANG Dong-mei, KANG Bo. Study on Differential Expression of OAZ1 and OAZ2 Gene in Various Tissues of the Sichuan White Goose[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 50-54. doi: 10.7668/hbnxb.2014.01.010.

http://www.hbnxb.net/CN/Y2014/V29/I1/50

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四川白鹅,i>OAZ1和OAZ2基因组织差异表达的研究

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