摘要： 利用电子克隆技术获得1个辣椒GH3同源基因的cDNA序列,采用生物信息学方法,对该基因所编码的氨基酸从理化性质、分子进化、跨膜结构域、高级结构等多角度进行分析。结果表明,该基因cDNA全长2 140 bp,其开放阅读框为1 791 bp,编码595个氨基酸。编码蛋白含32个磷酸化位点,无信号肽,无跨膜区域,是定位在叶绿体上的亲水性蛋白。通过氨基酸序列比对发现,辣椒GH3蛋白与拟南芥、大豆、烟草、番茄等植物中的GH3蛋白同源性较高。为该基因的基因功能研究提供了理论依据。

关键词: 辣椒, GH3基因, 电子克隆, 生物信息学

Abstract: A homologous gene of GH3 was obtained in Capsicum annuum by in silicon cloning, and some char-acters of the gene and its encoded protein were analyzed using the methods of bioinformatics in the following as -pects,including the general physical and chemical properties, hydrophobicity, three dimensional structure, subcellu-lar localization and phylogenetic relationships.The results showed the cDNA length of the gene was 2 140 bp long and contained a complete ORF ( 1 791 bp ) which encoded 595 amino acids.The deduced protein comprised 32 phosphorylation sites, no signal peptide and transmembrane helix.The GH3 of Capsicum annuum was a hydrophilic and chloroplast protein.The amino acid sequence of the gene showed high similarity with the homologous in other plants,such as Arabidopsis thaliana,Glycine max,Solanum lycopersicum,Nicotiana tabacum.The results laid a solid foundation for gene function analysis of GH3 in Capsicum annuum.

Key words: Capsicum annuum, GH3, In silico cloning, BioinfoRmatics

中图分类号: 

• S641.3