摘要： 为了调查浙江沿海地区副溶血弧菌的分布及其携带毒力基因的特征，在舟山、象山、温州三地实地采样385份，包括海水、泥土和水产品等，共分离到副溶血弧菌菌株278份，其中27株为tdh基因阳性，阳性率为9.7%，trh与ureC基因的阳性率分别为22.3%与11.8%，提示浙江沿海地区广泛存在着致病性副溶血弧菌污染。将不同来源副溶血弧菌的tdh和trh基因进行序列测定，进化树分析发现，环境分离株中tdh基因与临床分离株中tdh基因区分为2簇，而环境分离株与临床分离株中trh基因之间却没有明显的差异。研究结果可为浙江沿海地区副溶血弧菌的风险评估提供参考。
关键词:
副溶血弧菌,
环境分离株,
tdh基因,
trh基因,
ureC基因
Abstract: The tdh and trh genes from the isolates which were detected positive by PCR were furthercloned and sequenced phylogenetic analysis indicated that all the genes from seafoodelated samples were clus-tered in the same group，while the tdh genes from clinical isolates fell into a separate group. There were no significantdifferences of the trh genes between seafoodelated isolates and those from clinical source. The results gave the impor-tant reference for the V. parahaemolyticus risk assessment of Zhejiang province.
Key words:
Vibrio parahaerruicus,
Environmental strains,
tdh gene
中图分类号:
周向阳, 王淑娜, 周秀锦, 方维焕. 沿海地区副溶血弧菌的表型及其主要毒力基因分布特征[J]. 华北农学报, 2011, 26(1): 192-195. doi: 10.7668/hbnxb.2011.01.039.
ZHOU Xiang-yang, WANG Shu-na, ZHOU Xiu-jin, FANG Wei-huan. Vibrio parahaemolyticus Strains from Zhejiang Coastal Areas:Phenomena and Distribution of Major Virulent Genes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 192-195. doi: 10.7668/hbnxb.2011.01.039.