摘要： 本研究利用SX01、HB02两株根瘤菌菌株对山西右玉草地补植的紫花苜蓿中苜一号进行了田间接种试验。为了检验根瘤菌菌株的接种效果和筛选高效紫花苜蓿根瘤菌菌株，采用BOX-PCR分子标记方法对接种根瘤菌菌株的田间竞争结瘤能力进行了研究。通过对接种供试菌株及田间分离获得的根瘤菌菌株进行BOX-PCR及不同菌株之间的BOX分子指纹图谱比较，检测供试菌株的田间占瘤率。结果表明，紫花苜蓿接种供试菌株60d后，SX01、HB02两株根瘤菌菌株的田间占瘤率分别达到46.7%，53.3%，说明这两株根瘤菌菌株均具有较强竞争结瘤能力，可以作为高效根瘤菌菌株进行田间推广应用；并阐明了BOX-PCR作为一种分子标记方法，可对根瘤菌菌株竞争结瘤能力进行研究。
关键词:
BOX-PCR,
中苜－ 号,
根瘤菌,
占瘤率
Abstract: In this studytwo rhizobial strains SXO1 and HB02 were used to carry out the inoculation experimentwith reseeding Medicago sativa Zhongmu no.1 in You Yu county Shanxi Province. In order to test the effect of rhizo-bial strains inoculation and screen high efficient rhizobia strains，we used BOXCR Molecular Marker Method to Study the competitive nodulation ability of strain SXO1 and HB02.
Key words:
BOXCR,
Zhonmuno 1,
Rhizohia,
Nodule occupancy
中图分类号:
肖猛, 刘晓云, 刘桂霞, 戴燕燕, 郭振国, 郭晓叶, 魏爽. BOX-PCR分子标记对补播紫花苜蓿共生根瘤菌田间竞争结瘤能力的研究[J]. 华北农学报, 2011, 26(1): 187-190. doi: 10.7668/hbnxb.2011.01.038.
XIAO Meng, LIU Xiao-yun, LIU Gui-xia, DAI Yan-yan, GUO Zhen-guo, GUO Xiao-ye, WEI Shuang. Study on Competitive Nodulation Ability of Rhizobia in Symbiosis with ReseedingMedicago sativa in Field Test by Using BOX}CR Molecular Marker Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 187-190. doi: 10.7668/hbnxb.2011.01.038.