摘要： 采用RT-PCR方法从乌骨鸡皮肤中克隆出黑色素主效基因黑色素皮质激素受体(Melanocortin1-receptorMC1R)基因,长度为945bp,与普通白鸡比对其同源性为99.6%。以pEGFP-N1为基础,将从测序载体pMD-18T-MC1R上使用EcoR I和Sal I切下的MC1R连接到同样酶切的pEGFP-N1,构建pEGFP-N1-MC1R真核表达载体,为以后转基因动物的制备打下了基础。

关键词: 乌骨鸡, MC1R, 真核表达载体

Abstract: The MC1R gene(945 bp) was amplified with PCR from silky fowl' s skin. The sequence analysis demonstrated that the cloned MC1R of silky fowl shared 99. 6% homogeneity with MC1R gene of Gallus gallus. And the MC1R was digested from pMD-18T-MC1R to construct eukaryotic expressing vector with pEGFP-N1 plasmid.The constructed vectors provided a good background for transgene.

Key words: Silky fowl, MC1R, Eukaryotic expressing vector

中图分类号: 

• Q78