摘要： 为通过转基因技术改善草莓的贮运性能,以全明星草莓为试材,克隆了与草莓成熟有关的FaEtr2基因的2个片段,并构建了该基因的反义和正义植物表达载体。根据已克隆的草莓乙烯受体FaEtr2基因序列及表达载体pBI221上的酶切位点设计2对带限制性内切酶位点的特异性引物,以测序质粒为模板,PCR扩增到2个全明星草莓FaEtr2基因片段。两片段经双酶切消化后分别以正、反两个方向插入到植物表达载体pBI221的35 S启动子和NOS终止子之间,分别构建成All Star-FaEtr2基因的正、反义植物表达载体。

关键词: 草莓, 乙烯受体, FaEtr2基因, 植物表达载体

Abstract: Two fragments of All-star strawberry FaEtr2 gene were cloned using All-star strawberry as materials.Sense and antisense expression vector were constructed for improving storage and transport varieties of strawberry through transgenic technology.According to the restriction enzyme sites of expression vector and cloning sequence of strawberry FaEtr2 gene, two pairs of primers containing restriction enzyme sites were designed and used to amplify sequenced plasmid.PCR products and the plasmid pBL221 were digested by the corresponding restricted enzymes respectively, and linked directionally.Then the sense and antisense expression vector can be designed.The constructed expression vector was transformed into Agrobacterium LBA4404 for the following-up research.

Key words: Strawberry, Ethylene receptor, FaEtr2 gene, Plant expression vector

中图分类号: 

• S668.4