摘要： 黄瓜枯萎病是严重危害黄瓜生产的一种世界性土传病害,建立黄瓜枯萎病接种方法,从而实现快速、准确地对黄瓜种质资源进行抗病性筛选,明确不同黄瓜种质材料对枯萎病的抗性水平,可为黄瓜抗病育种提供重要依据.试验选用份抗感枯萎病黄瓜材料及其杂交后代,以危害我国黄瓜生产的优势枯萎病生理小种4为供试菌源,开展了苗期人工接种枯萎病抗性鉴定方法以及枯萎病抗性遗传规律研究.结果表明,浸根接种法,以1×106孢子/mL的接种液接种子叶期幼苗,在白天24～28℃,夜晚16～20℃的温室中培养,接种后10～14 d即可正确区分品种之间的抗感性差异.该方法发病迅速,整齐度、重复性好,是较理想的黄瓜枯萎病接种方法.运用浸根接种法对以WIS277和津研2号为双亲构建的F1、F2、F4 2个株系的各世代接种结果表明,WIS277抗枯萎病,津研2号感枯萎病,F1群体抗枯萎病.根据F2群体和F4株系的抗病表现推断WIS277对黄瓜枯萎病的抗性符合显性单基因的遗传模式.同时通过对Cu13和京育202及其F1的接种鉴定表明,Cu13抗枯萎病,京育202感枯萎病,F1群体表现中抗,初步认为抗病材料Cu13由多基因调控.
关键词:
黄瓜,
枯萎病,
接种方法,
抗性遗传
Abstract: Fusarium oxysporum f. sp. cucumerinum is one of the most destructive diseases on cucumber (Cucumis sativus L.) throughout China and the world. This experiment was carried out to establish a more reliable screening method and studied on the inheritance of resistance to Fusarium wilt race 4 for resistance breeding of cucumber. The result indicated that the best inoculation method was that seedling roots were dipped into inoculum suspension with 1 × 106 spores /mL when two cotyledons were fully expended，then incubating seedlings at 24－28℃ in daytime，16－20℃ at night in greenhouse. Plants are rated as resistant or susceptible 10－14 days after inoculation. The cucumber line WIS2757(parent 1) and the inbred line of Jinyan No. 2(parent 2) and F2 population and 52 F4 lines from the cross of both parents were employed for evaluation of the resistant to Fusarium wilt race 4. Parent WIS2757 plants and F1 plants were resistance to Fusarium wilt race 4，and parent Jinyan No.2 was susceptible. The segregating ratio of F2 population and F4 lines corresponded with the expected ratio at the 5% level of probability. And the cucumber line Cu13(parent 3) and Jinyu No. 202(parent 4) and F1 lines from the cross of both parents were employed for evaluation of the resistance to Fusarium wilt race 4. Parent Cu13 plants were resistance to Fusarium wilt race 4，parent Jinyu No. 202 was susceptible. F1 plants showed moderate resistance to Fusarium wilt race 4. The above results showed that the resistance of WIS2757 to Fusarium wilt race 4 was controlled by a single dominant gene and the resistance of Cu13 to Fusarium wilt race 4 may be controlled by multiple genes and the further study will be done in the future.
Key words:
Cucumber (Cucumis sativus L.),
Fusarium oxysporum f. sp.Cucumerinum,
Inoculation method,
Inheritance of resistance
中图分类号:
周红梅, 毛爱军, 张丽蓉, 张峰, 王永健, 杨文才. 黄瓜枯萎病接种方法及抗性遗传的研究[J]. 华北农学报, 2010, 25(4): 186-190. doi: 10.7668/hbnxb.2010.04.039.
ZHOU Hong-mei, MAO Ai-jun, ZHANG Li-rong, ZHANG Feng, WANG Yong-jian, YANG Wen-cai. Research on Inoculation Method and the Inheritance of Resistance to Fusarium oxysporum f.sp.cucumerinum on Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 186-190. doi: 10.7668/hbnxb.2010.04.039.