摘要： 从河南省有呼吸道症状的5个不同猪场采集棉拭子和肺组织样品.样品处理后,通过尿囊腔途径接种10日龄SPF鸡胚.获得3株病毒,经血凝和血凝抑制试验鉴定,为H3N2亚型流感病毒,并对其部分生物学特性进行了研究.结果表明,其半数鸡胚感染量(EID50)分别是0-9.56/0.2 mL,10-8.56/0.2 mL 和10-8./0.2 mL,对小白鼠的半数致死量(LD50)分别是10-1.56/0.1 mL,10-1.5/0.1 mL 和 10-1.50/0.1 mL;它们的血凝素对热不稳定,能凝集1%马、牛、驴、猪、绵羊、鸡、兔、豚鼠、小白鼠及人O型血的红细胞.这为了解河南猪群中流行的流感病毒奠定了基础.

关键词: 猪流感病毒, 分离, 鉴定, 生物学特性

Abstract: Samples were collected by cotton swabs and lung tissues from pigs with respiratory system problems on 5 farms in Henan province. Samples were inoculated into 10-day-old SPF-embryonated eggs via the allantoic route，after being processed. Three isolates were obtained and identified as influenza A virus subtype H3N2 by HA and HI. Some biological properties of them were studied. 50% egg infective doses (EID₅₀) were 10 ^{－9. 56} /0. 2 mL，10 ^{－8. 56} /0. 2 mL and 10 ^{－8. 44} /0. 2 mL. 50% lethal doses (LD₅0) to small white mice of the strain were 10 ^{－1. 56} /0. 1 mL，10^{－1. 45} /0. 1 mL and 10 ^{－1. 50} /0. 1 mL ，respectively. Hemagglutinins of the strains were unstable to heat. Red blood cells of horse， cattle，pig，donkey，pig，sheep，cock，rabbit，guinea pig，small white mouse，big white mouse and human O-type could be agglutinated by the isolate. It will lay a foundation for understanding influenza viruses circulating in swine herds in Henan province.

Key words: Swine influenza virus, Isolation, Identification, Biological properties

中图分类号: 

• S32.+1