华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 25-29. doi: 10.7668/hbnxb.2010.04.005

所属专题: 小麦 生物技术

• 论文 • 上一篇    下一篇

TcLr35小麦β(1,3;1,4)葡聚糖苷酶基因cDNA全长的克隆及分析

王海燕, 刘大群, 杨文香, 李在峰, 张立荣, 李星   

  1. 河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心, 河北 保定 071001
  • 收稿日期:2010-07-03 出版日期:2010-08-28
  • 通讯作者: 刘大群(1958- ),男,河北石家庄人,教授,博士,主要从事分子植物病理学研究。
  • 作者简介:王海燕(1977- ),女,河北遵化人,副教授,博士,主要从事分子植物病理学研究.
  • 基金资助:
    河北省自然科学基金项目(C2008000281;2010000702);河北省教育厅课题(Z2007408;2009130);国家自然科学基金(30700505)

Cloning and Characterization of β(1,3;1,4)Beta Glucanase Gene from TcLr35 Wheat

WANG Hai-yan, LIU Da-qun, YANG Wen-xiang, LI Zai-feng, ZHANG Li-rong, LI Xing   

  1. Biological Control Center of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
  • Received:2010-07-03 Published:2010-08-28

摘要: 根据已发表的植物β(1,3;1,4)葡聚糖苷酶基因设计引物,利用RT-PCR和RACE技术,从被小麦叶锈菌诱导的小麦抗叶锈病基因近等基因系材料TcLr3中获得一个β(1,3;1,4)葡聚糖苷酶基因cDNA全长,命名为PR34,该基因全长序列为1 416 bp,具有一个编码33个氨基酸的开放阅读框(ORF),其起始密码子ATG位于13 bp处,终止密码子TAG位于1 01 bp处,3′末端有20 bp的poly(A)尾,379 bp的3′非翻译区.与GenBank中小麦、大麦等多个葡聚糖苷酶基因具有较高同源性;对其推断的氨基酸序列进行分析,含有Glyco_hydro_17保守结构域,为葡聚糖苷酶基因蛋白结构域.Southern杂交显示,该基因在TcLr3小麦基因组中为低拷贝.

关键词: 小麦, &beta, (1, 3, 1, 4)葡聚糖苷酶, 基因克隆, 序列分析

Abstract: One pair of primer was designed based on the β(1,3; 1,4) beta glucanase,a full length wheat β(1,3;1,4) beta glucanase gene was obtained by RT-PCR and RACE from the RNA of TcLr35 carrying the Lr35 gene conferring resistance against wheat leaf rust induced by the Puccinia triticina named PR34,which was 1 416 bp in length, and included one complete open reading frame encoding 335 amino acids,a 379 bp 3' untranslated region(3' UTR) and 29 bp poly(A) tails. The initiation codon and stop codon were found in 13 bp locus and 1 015 bp locus respectively. The deduced amino acid sequence showed close homology to beta glucanase proteins which have been isolated from wheat, barley,and many other plants. The deduced amino acids contained Glyco_hydro_17 conserved domains which was related to beta glucanase. Southern blot indicated that the TcLr35 genome contained low copies of PR34 gene.

Key words: Wheat, β(1, 3, 1, 4) beta glucanase, Gene cloning, Sequence analysis

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引用本文

王海燕, 刘大群, 杨文香, 李在峰, 张立荣, 李星. TcLr35小麦β(1,3;1,4)葡聚糖苷酶基因cDNA全长的克隆及分析[J]. 华北农学报, 2010, 25(4): 25-29. doi: 10.7668/hbnxb.2010.04.005.

WANG Hai-yan, LIU Da-qun, YANG Wen-xiang, LI Zai-feng, ZHANG Li-rong, LI Xing. Cloning and Characterization of β(1,3;1,4)Beta Glucanase Gene from TcLr35 Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 25-29. doi: 10.7668/hbnxb.2010.04.005.

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