摘要： 采用不同浓度的山梨醇-硼酸络合物(SB)和硼酸(BA)处理,以不加硼为对照,研究其对小麦幼苗各器官硼含量、SOD、POD及PPO活性、可溶性蛋白含量以及叶绿素荧光参数的影响。结果表明:随培养液中硼浓度的升高,小麦幼苗根、叶鞘、叶片中硼含量显著升高。SB较BA更能提高小麦幼苗根及叶片中硼浓度。缺硼能引起SOD、POD和PPO活性的升高,加两种硼都能显著降低其活性,而SB在较低浓度(0.1,1.0μmol/L)时就表现出较好效果。小于20μmol/L时,ΦPSII、qP和ETR值随硼浓度上升而增大,SB处理增幅更显著,20μmol/L时又有所下降,可能是由于硼过量引起的。推测SB相对于BA更有利于小麦幼苗的吸收利用,在体内移动性更强。

关键词: 山梨醇-硼酸复合物, 硼酸, 保护酶, 叶绿素荧光, 小麦幼苗

Abstract: The effects of increasing levels(0,0.1,1.0,10 and 20 μmol/L)of sorbitol-boric acid(SB)and boric acid(BA)on the boron concentrations,the activities of major antioxidant enzymes(superoxide dismutase,SOD;peroxidase,POD,and polyphenoloxidase,PPO),soluble protein content and chlorophyll fluorescence parameters(Fo,Fm,Fv,Fo',Fm',Fv',qP,ΦPSII,ETR and NPQ)of wheat(Triticum aestivum L Huaimai 19)seedlings were investigated.The results showed that B content in roots,vagina and leaves of wheat seedlings increased significantly with the increasing concentrations of B in culture solution.B content in roots and leaves treated with SB increased more than that treated with BA.And B deficiency resulted in the increase of SOD,POD and PPO activity.The two kinds of B could significantly reduce their activities.And the reduction was significant in treatments with lower concentrations(0.1,1.0 μmol/L)of SB.When the concentration of B in culture solution was less than 20 μmol/L,the quantum yield of electron transfer at PSII(ΦPSII),photochemical quenching coefficient(qP)and electron transfer rate(ETR)values increased with the increase of it.These parameters increased more significantly in the treatment with SB.But at 20 μmol/L,they declined perhaps due to B excess.So we speculated that compared to BA,SB was more advantageous to the absorption and utilization by wheat seedlings and translocation in body more easily.

Key words: Sorbito-l boric acid comp lexes, Boric acid, Antiox idant enzymes, Ch lorophyll fluorescence, Wheat seed lings

中图分类号: 

• S512.01