摘要： 为了获得紫色大白菜细胞质不育系的特异序列,鉴定该不育系所属的不育类型,应用设计的 orF138上、下游引物PCR扩增6份材料:紫色大白菜CMS不育系,保持系07-721,杂交F1,3个回交BC1单株BC1-1、BC1-2和BC1-3.对获得的差异片段进行克隆、测序,同源性比较.结果表明,在紫色大白菜CMS不育系、杂交F1、3个回交BC1单株上均扩增到309 bp的特异产物,而保持系07-721没有扩增产物.测序和同源性比对结果表明,紫色大白菜CMS的orF138与萝卜Ogura不育系的orF138、芸薹属作物与萝卜Ogu CMS体细胞杂种的orF138同源性均达到99%,E值为2e-1.证明了紫色大白菜CMS不育系为一种改良的Ogura萝卜细胞质不育系,同时初步探讨了该不育系和紫色基因的应用前景.
关键词:
紫色大白菜,
Ogu CMS,
orF138,
应用
Abstract: In order to identify and certify the genotype of a purple Chinese cabbage CMS, and use the purple character - istics in Chinese cabbage, orF138 primerswere used for PCR analysis in 6materials including purple Chinese cabbage CMS, maintainer line 07 -721, F1, 3 back -crossing segregating plants BC1 -1, BC1 -2, BC1 -3.Then the PCR products were cloned, se - quenced and aligned.Results showed 309bp fragments could only be amplified in purple Chinese cabbage CMS, F1, BC1 -1, BC1 -2, BC1-3, excluding maintainer line 07 -721. Alignment results showed orF138 in purple Chinese cabbage CMS was ho - mologous to Ogu CMS in Raphanus sativus and cybrids of Brassica crops with Ra phanus sativus.The identities among them were 99%. E value was 2e -155. Therefore, the purple Chinese cabbage CMS material belongs to an improved Ogu CMS genotype.The application of the CMS genotype and purple genes in the future is discussed.
Key words:
Purple Chinese cabbage,
Ogu CMS,
orF138,
Application
中图分类号:
张德双, 陈斌, 张凤兰, 余阳俊, 赵岫云, 于拴仓, 徐家炳. 一种紫色大白菜细胞质不育系的分子鉴定[J]. 华北农学报, 2009, 24(3): 174-178. doi: 10.7668/hbnxb.2009.03.037.
ZHANG De shuang, CHEN Bin, ZHANG Feng lan, YU Yang jun, ZHAo Xiu yun, YU Shuan cang, XU Jia bing. Molecular Identification of A Purple Chinese Cabbage CMS Material[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 174-178. doi: 10.7668/hbnxb.2009.03.037.