摘要： 采用ELISA、PCR、RT-PCR及细胞培养等方法对山东某猪场流产、死胎及出生后几天死亡的小猪进行猪瘟病毒(CSFV),伪狂犬病毒(PRV),圆环病毒2型(PCV-2),细小病毒(PPV)和猪繁殖与呼吸综合征病毒(PRRSV)五种繁殖障碍性疾病进行检测,并应用ELISA猪瘟抗原检测试剂盒检测猪瘟病毒.检测结果表明,猪瘟病毒与圆环病毒2型为阳性,猪繁殖与呼吸综合征病毒、细小病毒、伪狂犬病毒均为阴性.根据结果判定该猪场发生猪瘟病毒与圆环病毒2型混合感染的繁殖障碍性疾病,其中以猪瘟感染最为严重.
关键词:
繁殖障碍,
PCR,
猪瘟,
圆环病毒2型,
诊断
Abstract: Aborted,fatal death and died after postnatal′ s pigletswere collected in Shandong province. The PCR,RT- PCR,ELISA tests and cell culture were performed to detect Porcine Reproductive Disturbance:classical swine fever virus ( CSFV), pseudorabies virus( PRV), porcine circovirus ( PCV), porcine parvovirus ( PPV)and porcine reproductive and respiratory syndrome virus ( PRRSV). From the detection results,CSFV,PCV-2 were positive,PRRSV, PRV, PPV were negative. The result indicated that the swine field were infected by CSFV and PCV-2,but CSFV was severity.
Key words:
Porcine reproductive disturbance,
PCR,
CSFV,
PCV- 2,
Diagnosis
中图分类号:
吴素丽, 孙惠玲, 张平, 张培君, 钱永华, 高立娜. 山东某猪场繁殖障碍性疾病的诊断研究[J]. 华北农学报, 2009, 24(3): 92-95. doi: 10.7668/hbnxb.2009.03.020.
WU Su li,, SUN Hui ling, ZHANG Ping, ZHANG Pei jun, QIAN Yong hua, GAo Li na. Diagnosis Research of Porcine Reproductive Disturbance in Shandong Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 92-95. doi: 10.7668/hbnxb.2009.03.020.