摘要： 减数分裂是有性繁殖的重要过程。已经发现,在玉米和拟南芥中,同源联会缺乏基因1(phs1),只允许同源染色体联会,而阻止非同源染色体联会。为了研究phs1在拟南芥中的表达,从拟南芥基因组中通过PCR扩增出phs1启动子,然后将phs1及其启动子克隆到含荧光表达蛋白GFP、YFP的表达载体上,最后将构建好的载体转化到根癌农杆菌中,用来转化拟南芥。
关键词:
减数分裂,
联会,
phs1,
荧光蛋白,
拟南芥
Abstract: Meiosis is crucial in sexual reproduction. It has been shown that poor homologous synapeis 1(phsl)ire-quired for homologous clmomosome pairing and for prevent synapsis between nonhomologous chromosomess. Promoter ofphsl was isolated by PCR from Arabidcpsas genome in order to study the copression of phsl,thereafter phsl and its promotor were cloned onto copression vector including green fluorescent protein and yellow fluorescent protein. At last, corerstructed vectors were transformed into agrobacterlum so that the vectors are to be transformed into Arabadcpsis.
Key words:
Meiosis,
Synapsis,
phsl,
Fluorescent protein,
Arabadopsas
中图分类号:
赵军良, 巫东堂, 王秀英, 李改珍, 巫一伦. 同源联会缺乏基因1启动子的分离及表达载体的构建[J]. 华北农学报, 2009, 24(1): 97-102. doi: 10.7668/hbnxb.2009.01.022.
ZHAO Jun-liang, WU Dong-tang, WANG Xiu-ying, LI Gai-zhen, WU Yi-lun. Isolation of Poor Homologous Synapsis 1 Promoter and Construction of Its Expressing Vector in Arabidopsis tlucliaha[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 97-102. doi: 10.7668/hbnxb.2009.01.022.