摘要： 研究Ca²⁺.CaM是否参与小麦抗叶锈病反应的过程,为更深入了解小麦抗叶锈病的反应机制奠定基础。采用离体培养的方法测定小麦种子经CaM拮抗剂TFP(三氟拉嗪)、CPZ(氯丙嗪)和W-7(N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide)以及CaCl2预处理后小麦叶片接种叶锈菌后在不同时间点的酶活性。另外用实时定量PCR的方法检测小麦接种亲和小种和非亲和小种后CaM不同亚型基因的表达情况。试验结果表明:小麦叶片接种非亲和性叶锈菌后,TFP、CPZ和W-7浸种处理抑制了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升;小麦叶片接种亲和性叶锈菌后,CaCl2预处理加剧了过氧化物酶(POD)、丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和过氧化氢酶(CAT)活性的上升。这说明Ca2+.CaM信使系统可能在小麦抗叶锈病过程中起着重要作用,并且不同的CaM亚型可能调控不同的抗病途径。

关键词: 小麦, 叶锈菌, Ca²⁺·, CaM

Abstract: Investigate if CaM is involved in the defense responses of wheat to Puccinia triticina, and provide more evidences to the next research. After being blocked Ca²⁺CaM messenger transduction by pretreating wit CaMantagonistsTFP(trifluoperazine),CPZ(chlorpromazine)and W-7 [ (N-(6-aminohexyl)-5-chloro-1-naphathalene sulfonamide)]and activated with pretreatment ofCaCl²⁺, the activities of peroxidase(POD), phenylalanine ammonialyade(PAL), polyphenoloxidase(PPO)and catalase(CAT)were measured in wheat leaves inoculated with Puccinia triticica.Addition, the expressionof the CaM gene was tested by Real-Time Fluorescent Quantitative PCR after inoculating virulent race and avirulentrace respectively.The results showed that the pretreatment with CaM repel reagents TFP,CPZ and W-7 inhibited theincrease in the activities of POD, PAL, PPO and CAT after the inoculation with 05-5-127 ③in detached wheat leaves.Thepretreatment with CaCl² enhanced the increase in the activities of POD, PAL, PPO and CAT after the inoculation with 05-22-64 ①in detached wheat leaves.And there is differential expression of CaM isforms genes under different interactions in wheat after inoculation.The results indicated that Ca²⁺·CaM messenger might play an important role in the interaction of wheat and pathogen, and different CaM isforms might play different role.

Key words: Wheat, Puccinia triticina, Ca²⁺·CaM

中图分类号: 

• S435.12