抑制性差减杂交技术(SSH)在植物学研究中的应用

doi:10.7668/hbnxb.2008.S2.017

摘要/Abstract

摘要： 抑制性差减杂交是一种基于转录水平的杂交技术,能将差异表达基因扩增千倍后富集,具有高效、灵敏、操作简单,假阳性率低等特点,已经越来越多的被应用在植物学研究领域。就该技术的原理、特点及其在植物学方面的应用研究进展作一简要介绍。

Abstract: Suppression subtractive hybridizat ion( SSH) is a hybridization technique based on transcriptional level, it could identify and collect different expressed genes by amplify the different genes over 1000 times. Because of the advan??tage of the technique including high efficiency, high sensitiveness, simple operation and low false positive rate, its applica??tion in plant is broadly more and more.This paper reviewed its working foundation, characters and application in plant re??search.

Key words: SSH, Different expressed genes, Botany

中图分类号:

阎爱华, 王冬梅. 抑制性差减杂交技术(SSH)在植物学研究中的应用[J]. 华北农学报, 2008, 23(S2): 78-83. doi: 10.7668/hbnxb.2008.S2.017.

YAN Ai-hua, WANG Dong-mei. Application of Suppression Subtractive Hybridization Technique in Plant Field[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(S2): 78-83. doi: 10.7668/hbnxb.2008.S2.017.

http://www.hbnxb.net/CN/Y2008/V23/IS2/78

参考文献

[1] Stolovitzky G A,Kundaje A,Held G A,el al.Statistical analysis of MPSS measurements:Application to the study of LPSactivated macrophage gene expression[J].Proc Natl Acad Sci USA,2005,102(5):1402-1407.
[2] Denis V R,Olga V B,Nadya G G,et al.Mirror orientation selection (MOS):a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization[J].Nucleic Acids Research,2000,28(20):e90.
[3] Yang G P,Ross D T,Kuang W W,et al.Combining SSH and cDNA microarrays for rapid identification of differentially espressed genes[J].Nucleic Acids Research,1999,27:1517-1523.
[4] Luo J H,Puc J A,Slosberg E D,et al.Differential subtraction chain,a method for identifying differences in genomic DNA and mRNA[J].Nucleic Acids Research,1999,27:e24.
[5] Li L,Techel D,Gretz N,el al.A novel transcriptome subtraction method for the detection of differentially expressed genes in highly complex eukaryotes[J].Nucleic Acids Research,2005,33(16):e136.
[6] Zhao Z,Huang X,Li N,et al.Direct cloning of cell differential expression genes with full-length by a new strategy based on the mutiple rounds of "long distance" polymerase chain reaction and magnetic beads mediated subtraction[J].J Biotechnol,1999,73(1):35-41.
[7] Kin J Y,Chuang Y S,Paek K H,et al.Isolation and characterization of a cDNA encoding the cysteine proteinase inhibitor,induced upon flower maturation in carnation using suppression subtractive hybridization[J].Mol Cells,1999,9 (4):392-397.
[8] Wang M L,Hsu C M,Chang L C,el al.Gene expression profiles of cold stored and fresh pollen to investigate pollen germination and growth[J].Plant Cell Physiol,2004,45 (10):1519-1528.
[9] Zhao Y H,Wang G Y,Zhang J P,et al.Expressed sequence tags(ESTs) and phylogenetic analysis of floral genes from a paleoherb species,Asarum caudigerum[J].Annal Bot,2006,98(1):157-163.
[10] Terefe D,Tatlioglu T.Isolation of a partial sequence of a putative nucleotide sugar epimerase,which may involve in stamen development in cucumber (Cucumis sativus L.)[J].Theor Appl Genet,2005,111(7):1300-1307.
[11] Bassani M,Neumann P M,Gepstein S.Differential expression profiles of growth related genes in elongation zone of maixe primary roots[J].Plant Mol Biol,2004,56(3):367-380.
[12] Bouton S,Viau L,Lelievre E,et al.A gene encoding a protein with a proline rich domain(MtPPRD1),revealed by suppressive subtractive hybridization (SSH),is specifically expressed in the Medicago truncatula embryo axisduring germination[J].J Exp Bot,2005,56(413):825-832.
[13] 姜大刚,卢森,周海,等.用EST和SSR标记定位水稻温敏不育基因tms5[J].科学通报,2006,51(6):148-151.
[14] 李小艳,赵云,周云涛,等.甘蓝型油菜矮化突变体抑制消减cDNA文库的构建及初步分析[J].中国油料作物学报,2006,28(4):396-402.
[15] Sylvain L,Theo H,Jean L H,et al.Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L.[J].BMC Plant Biology,2007,7(27).
[16] 王桂风,高燕,杨立伟,等.杉木木材形成过程特异表达基因的鉴定与分析[J].遗传,2007,29(4):483-489.
[17] Feng J X,Ji S J,Shi Y H,el al.Analysis of five differentially expressed gene families in fastelongation cotton fiber[J].Acta Biochim Biophys Sin,2004,36(1):51-56.
[18] Abebe T,Sadsen R W,Kaeppler H F,et al.Cloning and identification of highly expressed genes in barley lemma and palea[J].Crop Sci,2004,44:942-950.
[19] Yao Y Y,Ni Z F,Zhang Y H,et al.Identification of differentially expressed genes in leaf and root between wheat hybrid and its parental inbreds using PCR-based cDNA subtraction[J].Plant Mol Biol,2005,58(3):367-384.
[20] Mathews H,Clendennen S K,Caldwell C G,et al.Activation tagging in tomato identifies a transcriptional regulator of anthocyanin biosynthesis,modification and transport[J].Plant Cell,2003,15:1689-1703.
[21] 邓柳红,罗明武,曾会才,等.用抑制消减杂交法分离巴西橡胶树胶乳特异表达基因[J].林业科学,2006,42(6):32-36.
[22] 高雪,李正国,樊晶,等.奉节脐橙果皮褐变差减文库的构建及初步分析[J].植物生理与分子生物学学报,2007,33(1):71-76.
[23] Mercke P,Kappers I F,Verstappen F W A,et al.Combined transcript andmetabolite analysis reveals genes involved in spider mite induced volatile formation in cucunlber plants[J].Plant Physiol,2004,135:2012-2024.
[24] 罗志勇,陆秋恒,刘水平,等.人参植物皂昔生物合成相关新基因的筛选与鉴定[J].生物化学与生物物理学报,2003,35(6):554-560.
[25] 骆蒙,孔秀英,霍纳新.基于抑制消减杂交方法的小麦抗白粉病相关基因表达谱[J].科学通报,2002,47(16):1237-1241.
[26] 于秀梅,喻修道,屈志鹏,等.条锈菌诱导的小麦抑制差减杂交文库构建及其表达序列标签研究[J].植物病理学报,2007,37(1):50-55.
[27] 胡海燕,庄杰云,柴荣耀,等.水稻对不同小种稻瘟菌抗性差异表达基因的鉴定[J].中国水稻科学,2007,21(1):1-6.
[28] 刘同宝,卢建平,林福呈.稻瘟病菌附着胞差异表达基因文库的构建方法[J].植物病理学报,2006,36(2):129-134.
[29] Borras H O,Thomma B P,Collzao C,et al.EIL2 transcription factor and glutathione synthetase are required for defense of tobacco against tobacco blue mold[J].Mol Plant Microbe Interact,2006,19(4):399-406.
[30] Tian Z D,Liu J,Xie C H.Isolation of resistance relatedgenes to Phytophthora infestans with suppression subtractive hybridization in the R-gene-free potato[J].Acta Genetica Sinica,2003,30(7):597-605.
[31] Voiblet C,Duplessis S,Encelot N,et al.Identification of symbiosis regulated genes in Eucalyptus globulus Pisolithus tinctorius ectomycorrhiza by differential hybridization of arrayed cDNAs[J].Plant J,2001,25(2):181-191.
[32] Brechenmacher I,Weidmann S,Van T D,et al.Expression profiling of up regulated plant and fungal genes in early and late stages of Medicago truncatula-Glomus mosseae interaction[J].Mycorrhiza,2004,14 (4):253-262.
[33] 刘桂丰,侯英杰,王玉成,等.干旱胁迫下多枝柽柳正反向消减cDNA文库的构建[J].东北林业大学学报,2005,33(5):1-5.
[34] 林凡云.糜子干旱复水的基因表达谱分析及相关基因克隆[D].杨凌:西北农林科技大学,2006.
[35] 岳桂东,胡孝瑞,庄云龙,等.干旱胁迫下开花期玉米消减文库的构建及分析[J].山东大学学报,2006,41(4):168-172.
[36] Wang Z,Zang Q W,Guo Z A,et al.A preliminary study on gene expression profile induced by water stress in Wheat (Triticum aestivum L.)seedling[J].Acta Genetica Siniea,2004,31(8):842-849.
[37] 曾会明,沈听.胡杨(Populus euphratica)盐相关基因的克隆[J].内蒙古农业大学学报:自然科学版,2004,25(2):76-80.
[38] 张大栋,马鸿翔,吉晓佳,等.海蓬子盐胁迫初期抑制消减杂交cDNA文库的构建[J].江苏农业学报,2006,22(2):113-116.
[39] Bahn S C,Bae M S,Park Y B,et al.Molecular cloning and characterization of a novel temperature-induced gene,blti2,from barley(Hordeum vulgare L.)[J].Biochim Biophys Acta,2001,1522(2):134-137.
[40] Savenstrand,Brosche M,Strid A,et al.Regulation of gene expression by low levels of iultraviolet-B radiation in Pisum sativum:isolation of novel genes by suppression subtractive hybridization[J].Plant Cell Physiol,2002,43 (4):402 -410.
[41] Sahr T,Voigt G,Paretzke H G,et al.Caesium-affected gene expression in Arabidopsis thliana[J].New Phytol,2005,165(3):747-754.
[42] Watt D A.Aluminium-responsive genes in sugarcane:identification and analysis of expression under oxidative stress[J].J Exp Bot,2003,54(385):1163-1174.
[43] Zhang Y,Mian M A R,Chekhovskiy K,et al.Differential gene expression inFestuca under heat stress conditions[J].J Exp Bot,2005,56(413):897-907.
[44] 李同祥,王进科,王小兰,等.一种中药鉴定gDNA芯片的制备与展望[J].中草药,2005,36(7):961-964.
[45] 吕萌萌,王景安.低锌与正常锌条件下玉米基因表达差异的研究[J].华北农学报,2007,22(4):94-98.

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