摘要： 采用稀释平板分离法从张家界金鞭溪采集的土样中分离到20株放线菌。以烟草赤星病菌为靶标菌进行筛选,通过平皿对峙培养的方法,筛选出S-1,S-6,S-16,S-20等4株抑菌效果明显的菌株(抑菌率≥50%),抑菌率分别为51.7%,53.4%,51.9%,71.4%。进一步应用管碟法对菌株发酵液的代谢产物进行测定,结果表明,菌株S-20的抑菌圈直径为26.3 mm,抑菌带最宽,对烟草赤星病菌的抑制作用最显著。为了加深对该菌株的了解,本研究还对其形态特征、培养特征以及生理生化特性作了测定,初步鉴定菌株S-20为链霉菌属(Streptomycesspp.)的球孢类群(Globis-porus)。最后,抑菌谱试验表明,菌株S-20不仅对烟草赤星病菌作用明显,对其他病原真菌同样有抑制作用,抑菌谱广。

关键词: 烟草赤星病菌, 分离, 筛选, 鉴定

Abstract: Twenty Act inomyces were isolated from soil with dilution isolation, which collected from Jin bian brook,Zhang Jiajie. Antagonistic strains were screened for the bioact ivity targeted against Alternaria alternata. Pair-culturehowed S-1, S-6, S-16 and S-20 were screened which had higher inhibition rate(inhibition rate> 50% ). The inhibitionrate were 51.7%, 53.4%, 51.9%, 71.4%, and then the fermentation liquid of strains was determined, The resultsshowed S-20 had the best ant-i bacteria effect. The inhibition zones was 26??3mm, In order to recognize this strain, basedon its cultural, physiological and biochemical characterist ics, S-20 was designated as the series globisporus of stretomyce.The inhibition range showed that S-20 not only effect on Altermaria altermata but also on the other pathogens. The inhibition range widely.

Key words: Alternaria alternate, Isolation, Selection, Ident ification

中图分类号: 

• S435.72