摘要： 利用PCR方法检测vip1A/vip2A基因在野生型Bt菌株中的分布，进行基因片段的克隆和序列分析。结合cry1基因的PCR-RFLP基因型鉴定体系，分析与vip1A/vip2A基因有联系的cry1类基因。结果表明，分离保存的171株野生型Bt菌株中，有20株菌株含有vip1A/vip2A基因，分布率为11.69%。成功克隆了菌株Bt16的vip1A/vip2A基因片段，命名为vip1A/vip2A-16，GenBank 登录号为EU94327.cry1基因的PCR-RFLP鉴定结果表明，该20株菌株均含有cry1Fa基因，说明vip1A/vip2A基因与cry1Fa可能具有某种联系。
关键词:
二元毒素,
vip1A/vip2A,
vip1A/vip2A-16,
PCR-RFLP,
cry1Fa
Abstract: A PCR method and a PCR-restriction fragment length polymorphism method were used for identification ofvip1A/vip2A genes andcry1-type genes from Bacillus thuringiensis isolates,respectively.The result showed thatvip1A/vip2A-type genes appeared in 20 of 117 B.thuringiensis isolates.Avip1A/vip2A gene fragment was cloned and sequenced.GenBank accession number is EU594327 Thecry1-type genes were ident ified ascry1Fa.The results indicated that there was some linkage between thevip1A/vip2A andcry1Fa.
Key words:
Binary toxin,
vip1A /vip2A,
vip1A /vip2A-16,
PCR-RFLP,
cry1Fa
中图分类号:
申建茹, 郭巍, 张霞, 李晶. 苏云金杆菌vip1A／vip2A基因的鉴定及其与cry1基因的联系[J]. 华北农学报, 2008, 23(5): 131-135. doi: 10.7668/hbnxb.2008.05.029.
SHEN Jian-ru, GUO Wei, ZHANG Xia, LI Jing. Identification of thevip1A/vip2A Gene from Bacillus thuringiensis Isolates and the Linkage withcry1Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(5): 131-135. doi: 10.7668/hbnxb.2008.05.029.