摘要： 通过在卵母细胞体外成熟过程中添加不同浓度的卵丘细胞或其外围壁层颗粒细胞进行共培养,对山羊卵母细胞成熟过程中卵泡颗粒细胞对卵母细胞减数分裂抑制作用及作用机制进行了探索。结果表明,成熟液中添加10⁵和10⁶个/mL卵丘细胞或外围壁层颗粒细胞的卵母细胞体外成熟率与对照组相比差异不显著(p<0.05),但添加5×10⁶个/mL(41.2%)和10⁷个/mL(24.6%)卵丘细胞或外围壁层颗粒细胞组的成熟率显著降低(p<0.05);添加5×10⁶和10⁷个/mL卵丘细胞组和颗粒细胞组卵丘不能正常扩展,卵母细胞核状态观察,停留在GV期;Rp-cAMP(Rp-cyclicadenosine monophosphothioate)膜渗透性cAMP对抗剂,诱导了10⁷个/mL的卵丘细胞组(71.6%)和外围壁层颗粒细胞组(71.3%)的卵母细胞体外成熟率与对照组(72.0%)差异不显著(p<0.05)。在山羊卵母细胞体外成熟过程中,添加一定浓度的卵丘细胞及其外层的颗粒细胞可以提高卵母细胞胞质内的cAMP的水平,抑制卵母细胞减数分裂的启动,使卵母细胞停留在GV期。

关键词: 山羊, 卵泡颗粒细胞, 卵母细胞, 减数分裂, 抑制

Abstract: In this experiment,during the process of goat oocyte IVM,by adding different concentration cumulus cell and its surrounding granular cell,we studied the inhibitive effects and its mechanism of follicular cells on meiosis of oocytes. The IVM rate of oocyte was not being affected considerably after adding 10 ⁵ and 10⁶cell/ mL cumulus cells or surrounding granular cells,contrast to control group (p > 0.05),but were significantly higher than that of 5 × 10⁶cell/ mL(41.2 %),10⁷ cell/ mL(24.6 %)cumulus cells and 5 × 10⁶ cell/ mL(38.5 %),10⁷ cell/ mL(23.8 %) surrounding granu-lar cells(p < 0.05). In adding 5 × 10⁶,10⁷cell/ mL cumulus cells group and surrounding granular cells group,the cumu-lus could not expand.Nuclear Status Examination,the meiosis of oocytes was maintained in G V stage. Rp-cyclic adenosine monophosphothioate,a membrane permeable antagonist to cAMP,induced the oocyte IVM rate of 10⁷cell/ mL cumulus cells group (71.6 %)and surrounding granular cells group (71.3 %)which there was no significant difference among the control group (p > 0.05). during the process of oocyte IVM,adding some concentration cumulus cells and surrounding granular cells would maintain meiotic arrest by increasing the cAMP level of oocyte,make oocyte stay in the G V stage.

Key words: G oat, Follicular cell, Oocyte, Meiosis, Meiotic arrest

中图分类号: 

• S826