不同水系绒螯蟹线粒体DNA的遗传差异和群体遗传多样性研究

doi:10.7668/hbnxb.2008.03.014

摘要/Abstract

摘要： 对中国大陆6水系10个地区中华绒鳌蟹和合浦绒螯蟹自然群体及1个俄罗斯地区日本绒鳌蟹群体中共289个个体的线粒体细胞色素氧化酶亚基I进行了RFLP分析。选用8种能识别4碱基的限制性内切酶AluI,DpnII,HaeIII,MspI,MseI,RsaI,TaqI和TasI及3种能识别6碱基的限制性内切酶ApaI,NdeI和VspI对该基因片段酶切后,在11个群体中共检测到15种复合单倍型,其中复合单倍型1为除合浦绒鳌蟹以外的群体所共有,并均以该复合单倍型为主(46.4%~96.7%),复合单倍型4在合浦绒鳌蟹群体中占有很大比例(91.7%);合浦绒螯蟹与中华绒螯蟹北方水系(黄河、海河、辽河)的遗传差异最为明显(Pnet=0.060~0.061);不同水系中华绒螯蟹及合浦绒螯蟹、日本绒螯蟹具有一定的群体内遗传多态性(π=0.000 2~0.016 7),但并不高。

关键词: 绒鳌蟹, 遗传差异, 遗传多样性, 线粒体COI基因, 限制性片段长度多态性

Abstract: The genetic differences and diversity of mitten crab populations from 6 river systems in mainland China,1 population from Russia were studied based on RFLP analysis of mitochondrial cytochrome oxidase subunit I(COI)gene of 289 individual mitten crabs.The results showed that 15 composite haplotypes were detected among 11 populations after 11 restriction endonucleases(8 recognizing 4 base pairs and 3 recognizing 6 base pairs)digestion,while haplotype 1 was shared by all the samples except Eriocheir hepuensis and had high percentage(46.4%-96.7%),Eriocheir hepuensis was mainly composed of haplotype 4(91.7%)and had distinct genetic differences compared with Eriocheir sinensis from Yellow River,Haihe and Liaohe(Pnet=0.060-0.061);Genetic diversity of intrapopulation existed in Eriocheir sinensis of 6 River systems,Eriocheir hepuensis and Eriocheir japonica(π=0.000 2～0.016 7).

Key words: Mitten crab, Genetic deference, Genetic diversity, M itochondrial CO I gene, RFLP

中图分类号:

Q959.4

胡鹏飞, 李晓东, 戴伟, 王茜, 王晓梅. 不同水系绒螯蟹线粒体DNA的遗传差异和群体遗传多样性研究[J]. 华北农学报, 2008, 23(3): 50-55. doi: 10.7668/hbnxb.2008.03.014.

HU Peng-fei, LI Xiao-dong, DAI Wei, WANG Qian, WANG Xiao-mei. Genetic Differences and Diversity of Mitten Crabs Eriocheir from Different Rivers based on mtDNA Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(3): 50-55. doi: 10.7668/hbnxb.2008.03.014.

http://www.hbnxb.net/CN/Y2008/V23/I3/50

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