摘要： 以拟南芥幼苗总RNA为模板,采用RT-PCR技术扩增到DREB2A基因,并将其克隆到植物表达载体pCAM-BIA1304中CaMV 35 S启动子与poly(A)终止子之间。酶切鉴定及测序列结果都表明,成功构建了植物表达载体pCAMBIA1304-DREB2A。另外,获得了携带DREB2A基因的根瘤农杆菌菌株,为以后转基因植物工作奠定了基础。

关键词: 拟南芥, DREB2A, RT-PCR, 植物表达载体, 根瘤农杆菌

Abstract: In the paper,total RNA was extracted from Arabidcpsas thalaana seedlings and was used as template to amplify the DREB2A gene by RT-PCR.The gene fragment was subsequently cloned into plant expression vector pCAMBIA1304,located between CaMV35S promoter and poly(A)terminator.Restriction enzyme analysis and DNA sequencing confirmed that the plant expression vector pCAMBIA1304-DREB2A was successfully construed.In addition,the strain of Agrobacteraum twnefaciens carrying DREB2A gene were obtained,providing the foundation for transgenic study of plants.

Key words: Arabidcpsas thalaana, DREB2A, RT-PCR, PIant expression vector, Agrobacteraum twnefaciens

中图分类号: 

• Q78