摘要： 试验表明,芽孢杆菌B96-II对芦笋茎枯病菌(Phomopsis asparagiSacc)菌丝生长的抑制率为89.62%,对孢子产生的抑制率为99.65%;对芦笋茎枯菌的田间防治效果为93.10%。研究表明,B96-II的拮抗蛋白对芦笋茎枯菌有明显的抑制活性;对芦笋的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)均有激活和诱导作用。B96-II处理后30 d,芦笋组织的SOD活力较对照提高254.98%,CAT活力提高81.84%,POD活性提高22.13%,PPO活力提高146.67%,PAL活力提高171.60%。
关键词:
芦笋茎枯病,
芽胞杆菌,
拮抗蛋白,
诱导抗性,
保护酶
Abstract: Antagonistic Bacillus strain B96一II could effectively inhibite mycelia growth and spore germination ofPhomopsis asparagi Sacc,pathogen of asparagus stem blight. The inhibition rates were 89. 62%and 99. 65%respective-1y. Field trials showed a control efficacy of 93. 10%.Contents of 5 resistance related enzymes in asparagus plants werestimulated distinctly 30 d after treatment with B96一II. Cbmpared with untreated control sample,superoxide dismutase ac-tivity had an increase of 254. 98%,such as 81.84%to catalase,22. 13%to peroxidase,146. 67%to polyphenoloxidase，and 171. 60%to phenylalanin ammonialyase.
Key words:
Asparagus stem blight,
Bacillus sp,
Antagonistic protein,
Induced resistance,
Protective enzyme
中图分类号:
马利平, 郝变青, 秦曙, 乔雄梧. 芽孢杆菌B96-II对芦笋茎枯病的防治及机制研究[J]. 华北农学报, 2008, 23(2): 180-184. doi: 10.7668/hbnxb.2008.02.040.
MA Li-ping, HAO Bian-qing, QIN Shu, QIAO Xiong-wu. Causal Study on Inhibition Effects of Antagonistic Bacillus Strain B96-II to Asparagus Stem Blight[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(2): 180-184. doi: 10.7668/hbnxb.2008.02.040.