摘要： 利用农业上广泛使用的酰胺类除草剂甲基胺草磷,在1~4μmol/L浓度下,对野二燕1号小麦根尖分生组织细胞染色体结构变异及蛋白质组分变化进行了研究,结果发现,随着浓度升高,染色体结构变异率呈上升趋势。在3~4μmol/L浓度下处理的根尖分生组织细胞中,多极分裂细胞的出现频率为0.08%;染色体桥、断片和滞后染色体的出现频率为0.434%。双向电泳分析结果表明,约有10个蛋白质斑点产生变化,2个蛋白质斑点(8 kDa/PI 6.5,10kDa/PI 7.7)消失。染色体结构变异和蛋白质组分变化可以认为与甲基胺草磷的处理有关。

关键词: 甲基胺草磷, 染色体结构变异, 双向电泳, 蛋白质组分, 小麦

Abstract: The effect of phosphoric amide herbicide amiprophose2methyl(APM),which was used wildly in agricul2ture,on chromosome structure and protein composition of root meristems of wheat were studied by exposure to concentra2tions of 1 - 4μ mol/L. The results indicated that,as the concentration of APM increased the frequency of chromosomalstructure variation increased. Frequencies of multipolar division cells and bridges lagging chromosome were 0108 % and01434 % respectively when root meristems were treated with 3 - 4μ mol/L APM. 2D2PAGE analysis showed that about 10new protein spots were induced and of 2 protein spots(8 kDa/PI 615,10 kDa/PI 717)disappeared by 3 - 4μ mol/L APMin the root meristem. Chromosome structure variation and protein composition changes were thought to be directly relatedto APM treatment.

Key words: APM, Chromosome structure, 2D2PAGE, Protein composition, Wheat

中图分类号: 

• S48214+6