摘要： 通过在SMCS培养液中加入不同的碳源、氮源,改变培养时间、温度及培养液的pH值,研究了盾壳霉产葡聚糖酶的培养条件。结果表明:盾壳霉接种在改良的SMCS液体培养基中置于恒温摇床(200r/min,20℃)上培养15d,可以诱导产生大量的葡聚糖酶。改良的SMCS培养液配方为:KH2PO4680mg,K2HPO4870mg,KCl200mg,NH4NO31g,Mg-SO4.7H2O200mg,CaCl2200mg,FeSO42mg,ZnSO42mg,MnSO42mg,蔗糖10g,加水1000mL,调pH值到6.5。

关键词: 盾壳霉, 葡聚糖酶, 培养条件

Abstract: In this study through the different carbon source , the nitrogen source and the double carbon source are mixed in the SMCS nutrient fluid .The culturing time , the temperature and the pH are changed in order to study culturing condition of glucanases produced by Coniothyrium minitans .The result indicated that , Coniothyrium minitans on the improved SMCS nutrient fluid at the constant temperature on the skakier(200 r/min ,20 ℃)about 15 d ,may induce produces massive glucanases .The improved SMCS nutrient fluid formula is :KH₂PO₄ 680 mg ,KH₂PO₄ 870mg ,KCl 200mg ,NH₄NO₃ 1 g , MgSO₄·7H₂O 200mg ,CaCl₂ 200mg , FeSO₄ 2 mg , ZnSO₄ 2mg ,MnSO₄ 2mg , saccharose 10 g ,water 1 000mL , adjusting the pH to 6.5

Key words: Coniothyrium minitans, Glucanases, Culturing condition

中图分类号: 

• S476