一株苏云金芽孢杆菌的分离与杀虫基因的鉴定

doi:10.7668/hbnxb.2007.04.043

摘要/Abstract

摘要： 采用温度筛选从云南省刁苓山土壤中分离得到一株伴孢晶体为长菱形的苏云金芽孢杆菌BSH-1.利用PCR-RFLP鉴定体系和SDS-PAGE对菌株所含cry基因类型和晶体蛋白的构成进行鉴定、分析，结果表明：菌株BSH-1具有的cry基因组合为：cry1Aa，cry1Ac，cry1 Db，cry2 Ab，cry1 Ia，表达的晶体蛋白有130，70和6 kDa 3种，该蛋白对甜菜夜蛾具有较高杀虫活性，LC₅₀为1.43 ng/mg。该菌株的基因组合类型对构建杀虫基因工程菌具有重要参考价值。

关键词: 苏云金芽孢杆菌, 分离, 杀虫基因, 鉴定

Abstract: In this paper a Bacillus thuringiensis strain was isolated from the soil sample of Diaoling Mountain in Yunnan province. The cry gene-types of Bt isolate was identified by use of PCR-RFLP and SDS-PAGE methods. This strain which had a cry-type includes cry1Aa, cry1Ac, cry1Db, cry2Ab, cry1Ia produced Bipyramidal shape crystal, the protein composed of the crystal were 130, 70 and 56 kDa, and exhibited insecticidal activity against Laphygma exigua (first-instar), LC₅₀ was 1.43 ng/mg. It can be used in composing the bacteria of gene engineering.

Key words: Bacillus thuringiensis, Isolation, Insecticidal gene, Identification

中图分类号:

S432

苏旭东, 马雯, 马晓燕, 王雪静, 李英军, 檀建新. 一株苏云金芽孢杆菌的分离与杀虫基因的鉴定[J]. 华北农学报, 2007, 22(4): 184-188. doi: 10.7668/hbnxb.2007.04.043.

SU Xu-dong, MA Wen, MA Xiao-yan, WANG Xue-jing, LI Ying-jun, TAN Jian-xin. Isolation and Identification of a Bacillus thuringiensis Strian[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 184-188. doi: 10.7668/hbnxb.2007.04.043.

http://www.hbnxb.net/CN/Y2007/V22/I4/184

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