摘要： 通过醋酸钠-抗生素筛选法从河北省土壤中筛选得到了苏云金芽孢杆菌新菌株CY1.镜检可观察到伴孢晶体呈小菱形。SDS-PAGE分析结果表明菌株CY1表达的晶体蛋白分子量为130 kDa。利用25对通用引物检测cry1，cry11，cry7，cry2，cry3，cry4/cry10，cry5，cry6，cry7，cry8，cry9，cry11，cry13/1，cry16/17，cry18，cry19，cry21，cry22，cry2/25，cry26/28，cry27/29，cry30，cry32，cry3/35，cry40基因，仅从CY1菌株中检测出cry7基因，该cry7基因N末端的1215 bp片段所编码的氨基酸序列与已发表的cry7Ab1(Acc No.：U0367)序列同源性达99%，仅有2个氨基酸的差异。用3对cry7Ab特异引物检测，进一步证实该菌株携带有cry7Ab基因。
关键词:
苏云金芽胞杆菌,
CY1菌株,
cry7基因
Abstract: A novel strain CY1 of Bacillus thuringiensis (Bt) was isolated from the soil of Hebei province by Sodium acetate-antibiotic method. Bipyramidal inclusions could be visualized by microscope. SDS-PAGE analysis indicated that the strain producing a 130 kDa protein. The cry gene analysis of the strain was based on multiplex PCR with novel general primers that could detect the cry1, cry11, cry7, cry2, cry3, cry4/cry10, cry5, cry6, cry7, cry8, cry9, cry11, cry13/1, cry16/17, cry18, cry19, cry21, cry22, cry2/25, cry26/28, cry27/29, cry30, cry32, cry3/35, and cry40 genes. Only cry7 gene were found. PCR product of cry7 gene was sequenced. The cry7 gene fragments had 99% homology to cry7Ab1. The strains were further characterized to carry cry7Ab gene by additional PCRs with specific cry7Ab primers.
Key words:
Bacillus thuringiensis,
CY1 strain,
cry7 gene
中图分类号:
吴会贤, 宋萍, 王勤英, 苏旭东. 苏云金芽胞杆菌CY1菌株的cry基因分析[J]. 华北农学报, 2007, 22(4): 180-183. doi: 10.7668/hbnxb.2007.04.042.
WU Hui-xian, SONG Ping, WANG Qin-ying, SU Xu-dong. Characterization of cry Gene from a Novel Bacillus thuringiensis CY1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 180-183. doi: 10.7668/hbnxb.2007.04.042.