摘要： 以黄瓜为研究材料，研究了影响cDNA-AFLP反应体系的几个关键因素，建立了适宜黄瓜的cDNA-AFLP分析体系，并得到了较为清晰可辨的cDNA-AFLP指纹图谱。结果表明，适用于黄瓜dscDNA的酶切组合为Taq Ⅰ和Vsp Ⅰ，dscDNA酶切用量为300 ng，用作预扩增模板的连接产物稀释倍数为1倍，作为选择性扩增模板的预扩增反应产物的适宜稀释倍数为30倍。

关键词: 黄瓜, cDNA-AFLP, 分析体系

Abstract: After systematical studies on the factors affecting cDNA-AFLP analysis system of cucumber, an optimized system was established which showed a distinct electrophoretogram of cDNA-AFLP. The results indicated that the restriction enzyme combination was Taq I and Vsp I, the dosage of dscDNA was 300 ng, the ligation products using as template of pre-amplification were diluted to one time, and the pre-amplification products using as template of select-amplification were diluted to 30 times.

Key words: Cucumber, cDNA-AFLP, Analysis system

中图分类号: 

• S642.2